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2025 Vol.34, Issue 3 Preview Page

Original Articles

Growth Responses of Cucumber Seedlings to Single and Combined Stresses of Mechanical Stimulation and Salinity

기계자극과 염의 단일 및 복합 스트레스에 의한 오이묘의 생육 반응

Hyeon Woo Jeong1
,
Hee Sung Hwang2
,
Jin Yu2
,
Eun Won Park3
,
So Yeong Hwang4
,
Ji Hye Yun4
,
Jeong Kill Koo4
,
Hyeong Eun Choi4
,
Seong Jae Hwang5678*
정 현우1
,
황 희성2
,
유 진2
,
박 은원3
,
황 소영4
,
윤 지혜4
,
구 정길4
,
최 형은4
,
황 승재5678*
1Researcher, Protected Horticulture Researcher Institute, NIHHS, Haman 52054, Korea
2RDA Research Associate, Protected Horticulture Researcher Institute, NIHHS, Haman 52054, Korea
3Researcher, Medicinal Resources Research Institute, Gyeongsangnam-do Agricultural Research and Extension Services, Hamyang 50005, Korea
4Graduate Student, Division of Applied Life Science, Grduate School of Gyeongsang National University, Jinju 52828, Korea
5Researcher, Institute of Agriculture &. Life Sciences, Gyeongsang National University, Jinju 52828, Korea
6Professor, Division of Horticultural Science, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea
7Professor, Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea
8Professor, Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea
1농촌진흥청 국립원예특작과학원 연구사
2농촌진흥청 국립원예특작과학원 전문연구원
3경상남도 농업기술원 약용자원연구소 연구원
4경상국립대학교 응용생명과학부 대학원생
5경상국립대학교 농업생명과학연구원 연구원
6경상국립대학교 농업생명과학대학 원예과학부 교수
7경상국립대학교 농업생명과학연구원 교수
8경상국립대학교 생명과학연구원 교수
*Corresponding Author
31 July 2025. pp. 335-341
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Abstract

Earlier studies have reported that mechanical stimulation can enhance plant resistance to various environmental stresses. In this study, we evaluated the response of cucumber seedlings to salt stress combined with mechanical stimulation. Cucumber seeds were sown in 40-cell plug trays filled with commercial seedling medium. Brushing treatment using an acrylic film was applied every 2 hours, starting 29 days after sowing. After 7 days of brushing, NaCl solutions at concentrations of 50, 100, 150, and 200 mM were applied to both brushing and non-brushing groups. Growth and stress responses were measured 9 days after NaCl treatment. Plant height, leaf length, leaf width, leaf area, and fresh weights of shoots and roots decreased as NaCl concentration increased. However, SPAD values increased with higher NaCl levels. Brushing treatment reduced plant height regardless of NaCl concentration. While total root length and root volume decreased with increasing NaCl, brushing treatment mitigated these effects by increasing root length and root volume. MDA and H2O2 levels increased with NaCl treatment, and the brushing group showed higher contents of both than the non-brushing group. These results suggest that both mechanical and salt stresses increase stress responses in cucumber seedlings, and mechanical stimulation may alleviate salt-induced root growth inhibition. Therefore, it is suggested that stress tolerance may be induced through the regulation of the intensity of appropriate mechanical stimulation.

Keywords
brushing treatment
MDA
NaCl
root morphology
total root length

이전의 연구에서는 기계적 자극이 다양한 환경 스트레스에 대한 식물의 저항성을 향상한다고 보고된 바 있다. 본 연구에서는 기계적 자극과 염 스트레스에 대한 오이묘의 반응을 조사하고자 수행되었다. 오이 종자는 상업적 공정육묘상토가 충진된 40구 트레이에 파종하였고 파종 후 29일부터 아크릴 필름을 이용하여 브러싱 처리를 2시간 간격으로 진행하였다. 브러싱 자극 7일 후 NaCl 용액을 이용하여 50, 100, 150, 200mM의 농도로 처리하였다. NaCl 처리 9일 후 오이묘의 생육과 스트레스 반응을 조사하였다. NaCl의 농도가 증가함에 따라 초장, 엽장, 엽폭, 엽면적 그리고 지상부의 생체중이 감소 하였으며, SPAD 값은 NaCl의 농도와 관계없이 처리구에서 증가하였다. NaCl 농도가 증가함에 따라 총 근장과 뿌리 부피가 감소하였지만, 브러싱 처리구에서는 총 근장과 뿌리 부피가 증가하여 NaCl의 처리에 대한 영향을 완화 시켰다. MDA와 H2O2 농도는 NaCl의 처리에 의해 증가하였으며 브러싱 처리로 인해서도 높은 MDA와 H2O2 함량이 나타났다. 결론적으로 기계적 스트레스와 염분 스트레스가 모두 스트레스 반응을 일으켰으며, 기계적 자극이 염 스트레스로 인한 뿌리 생장 억제를 완화할 수 있음을 알 수 있다. 따라서 적절한 기계적 자극의 처리 강도 조절을 통한 스트레스 저항성을 유도할 수 있을 것으로 보여진다.

키워드
브러싱
MDA
NaCl
지하부 생육
총 근장
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Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Journal of Bio-Environment Control
  • Journal Title(Ko) :생물환경조절학회지
  • Volume : 34
  • No :3
  • Pages :335-341
  • Received Date : 2025-07-21
  • Revised Date : 2025-07-23
  • Accepted Date : 2025-07-25
  • DOI :https://doi.org/10.12791/KSBEC.2025.34.3.335
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