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2025 Vol.34, Issue 3 Preview Page

Original Articles

Growth and Photosynthetic Responses of Lettuce and Basil to Fluctuating Light Intensity in a Plant Factory

식물공장에서의 광도 변동에 따른 상추와 바질의 생장 및 광합성 반응

Jaeho Jeong1
,
Yurina Kwack2*
정 재호1
,
곽 유리나2*
1Graduate Student, Department of Environmental Horticulture, University of Seoul, Seoul 02504, Korea
2Professor, Department of Environmental Horticulture, University of Seoul, Seoul 02504, Korea
1서울시립대학교 자연과학대학 환경원예학과 대학원생
2서울시립대학교 자연과학대학 환경원예학과 교수
*Corresponding Author
31 July 2025. pp. 325-334
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Abstract

Light is a key environmental factor that strongly influences plant growth and physiology. In plant factories, light intensity is typically kept constant throughout cultivation. This study evaluated the effects of daily fluctuating light intensity on the growth and photosynthesis of lettuce (Lactuca sativa L.) and basil (Ocimum basilicum L.) under equivalent light integral conditions. At 25 days after sowing (25 DAS), plants were subjected to eight light treatments for seven days. The control maintained a constant light intensity of 250 µmol·m-2·s-1, while treatments L1－L7 involved daily fluctuations between 150 and 350 µmol·m-2·s-1. Photosynthetic responses in both species generally followed the light fluctuation patterns. Gradual increases in light intensity had no effect on stomatal conductance, whereas rapid increases significantly reduced it. At 32 DAS, the highest growth in lettuce was observed under the control and L5 (irregular fluctuations), whereas the highest growth in basil was observed under L2 (gradual increase followed by decrease). These results indicate that short-term modulation of daily light intensity prior to harvest can improve productivity, with species-specific responses to light fluctuation.

Keywords
biomass
LED
light integral
PPFD
stomatal conductance

대광은 식물의 생장과 생리에 큰 영향을 미치는 핵심 환경 요소이다. 식물공장에서는 일반적으로 재배 기간 동안 광도가 일정하게 유지된다. 본 연구는 동일한 누적 광량 조건 하에서 일일 광도 변동이 상추(Lactuca sativa L.)와 바질(Ocimum basilicum L.)의 생장 및 광합성에 미치는 영향을 평가하였다. 파종 후 25일(25 DAS)에 식물에 7일간 8가지의 광 처리(L1－L7)를 적용하였다. 대조구는 250 µmol·m-2·s-1의 일정한 광도를 유지하였고, L1부터 L7까지의 처리구는 150에서 350 µmol·m-2·s-1 사이의 일일 광도 변동을 수반하였다. 두 작물 모두에서 광합성 반응은 전반적으로 광도 변동 패턴을 따랐다. 광도가 점진적으로 증가하는 경우에는 기공전도도에 영향이 없었으나, 급격한 증가가 있을 경우에는 기공전도도가 유의미하게 감소하였다. 32 DAS 에는 상추에서 대조구와 L5 처리구(불규칙한 변동)에서 가장 높은 생장이 나타났으며, 바질에서는 L2 처리구(점진적 증가 후 감소)에서 가장 높은 생장이 관찰되었다. 이 결과는 수확 직전의 단기적인 일일 광도 조절이 생산성을 향상시킬 수 있으며, 그 반응은 작물별로 다르게 나타난다는 것을 시사한다.

키워드
기공 전도도
바이오매스
적산 광량
LED
PPFD
References
1

Anum H., R.F. Cheng, and Y.X. Tong 2024, Improving plant growth, anthocyanin production and oxidative status of red lettuce (Lactuca sativa cv. Lolla Rossa) by optimizing red to blue light ratio with a constant green light fraction in a plant factory. Sci Hortic 338:113832. doi:10.1016/j.scienta.2024.113832

10.1016/j.scienta.2024.113832
2

Cha M.K., J.S. Kim, and Y.Y. Cho 2012, Growth response of lettuce to various levels of EC and light intensity in plant factory. J Bio-Env Con 21:305-311.

10.12791/KSBEC.2012.21.4.305
3

Chang C.L., and K.P. Chang 2014, The growth response of leaf lettuce at different stages to multiple wavelength-band light-emitting diode lighting. Sci Hortic 179:78-84. doi: 10.1016/j.scienta.2014.09.013

10.1016/j.scienta.2014.09.013
4

Chen M., J. Chory, and C. Fankhauser 2004, Light signal transduction in higher plants. Annu Rev Genet 38:87-117. doi:10.1146/annurev.genet.38.072902.092259

10.1146/annurev.genet.38.072902.092259
5

Hang T., N. Lu, M. Takagaki, and H. Mao 2019, Leaf area model based on thermal effectiveness and photosynthetically active radiation in lettuce grown in mini-plant factories under different light cycles. Sci Hortic 252:113-120. doi:10.1016/j.scienta.2019.03.057 j.scienta.2019.03.057

10.1016/j.scienta.2019.03.057
6

He R., Y. Zhang, S. Song, W. Su, Y. Hao, H. Liu 2021, UV-A and FR irradiation improves growth and nutritional properties of lettuce grown in an artificial light plant factory. Food Chem 345:128727. doi:10.1016/j.foodchem.2020.128727

10.1016/j.foodchem.2020.128727
7

Kaiser E., P. Kusuma, S. Vialet-Chabrand, K. Folta, Y. Liu, H. Poorter, N. Woning, S. Shrestha, A. Ciarreta, J. van Brenk, M. Karpe, Y. Ji, S. David, C. Zepeda, X.G. Zhu, K. Huntenburg, J.C. Verdonk, E. Woltering, P.P.G. Gauthier, S. Courbier, G. Taylor, L.F.M. Marcelis 2024, Vertical farming goes dynamic: optimizing resource use efficiency, product quality, and energy costs. Front Sci 2:1411259. doi: 10.3389/fsci.2024.1411259. 1411259

10.3389/fsci.2024.1411259
8

Kelly N., D. Choe, Q. Meng, and E.S. Runkle 2020, Promotion of lettuce growth under an increasing daily light integral depends on the combination of the photosynthetic photon flux density and photoperiod. Sci Hortic 272:109565. doi:10.1016/j.scienta.2020.109565

10.1016/j.scienta.2020.109565
9

Kim Y.H. 1999, Plant Growth and Morphogenesis Control in Transplant Production System using Light-emitting Diodes (LEDs) as Artificial Light Source. J Biosyst Eng 24:115-122.

10

Kozai T. 2012, Sustainable plant factory: Closed plant production systems with artificial light for high resource use efficiencies and quality produce. Acta Hortic pp 27-40. doi:10.17660/ActaHortic.2013.1004.2 ActaHortic.2013.1004.2

10.17660/ActaHortic.2013.1004.2
11

Kozai T., G. Niu, and M. Takagaki 2019, Plant Factory: An indoor vertical farming system for efficient quality food production. Elsevier, London, UK, pp 11-22.

12

Larsen D.H., H. Li, S. Shrestha, J.C. Verdonk, C.C. Nicole, L.F. Marcelis, E.J. Woltering 2022, Lack of blue light regulation of antioxidants and chilling tolerance in Basil. Front Plant Sci 13:852654. doi:10.3389/fpls.2022.852654

10.3389/fpls.2022.85265435463427PMC9021895
13

Larsen D.H., E.J. Woltering, C.C.S. Nicole, and L.F.M. Marcelis 2020, Response of Basil Growth and Morphology to Light Intensity and Spectrum in a Vertical Farm. Front Plant Sci 11:597906. doi:10.3389/fpls.2020.597906

10.3389/fpls.2020.59790633424894PMC7793858
14

Mäkinen A., H. Ishihara, S. Poque, N. Sipari, K. Himanen, I. Varjus, J. Heininen, M. Pastell, P. Elomaa, A. Shapiguzov, T. Kotilainen, S. Kangasjärvi 2025, Photosynthetic adjustments maintain lettuce growth under dynamically changing lighting in controlled indoor farming setups. Physiol Plant 177:e70405. doi:10.1111/ppl.70405

10.1111/ppl.7040540650517PMC12254939
15

Mohamed S.J., H.Z. Rihan, N. Aljafer, and M.P. Fuller 2021, The impact of light spectrum and intensity on the growth, physiology, and antioxidant activity of lettuce (Lactuca sativa L.). Plants 10:2162. doi:10.3390/plants10102162

10.3390/plants1010216234685971PMC8538153
16

Niu Y., S. Matsubara, L. Nedbal, and D. Lazár 2024, Dynamics and interplay of photosynthetic regulatory processes depend on the amplitudes of oscillating light. Plant Cell Environ 47:2240-2257. doi:10.1111/pce.14879

10.1111/pce.14879
17

Park K.S. 2016, Development of photosynthesis and growth models of sweet basil and ice plant in plant factories. Doctoral dissertation, Graduate School, Seoul National Univ., Seoul. pp 28-44.

18

Saha S., A. Monroe, and M.R. Day 2016, Growth, yield, plant quality and nutrition of basil (Ocimum basilicum L.) under soilless agricultural systems. Ann Agric Sci 61:181-186. doi:10.1016/j.aoas.2016.10.001

10.1016/j.aoas.2016.10.001
19

Samy E.R.A. 2023, Assessing the effect of planting density on romaine lettuce growth and quality in a controlled hydroponic environment. Masteral dissertation, Graduate School, McGill Univ., Montreal, Quebec, Canada. pp 18-19.

20

Shafiq I., S. Hussain, M.A. Raza, N. Iqbal, M.A. Asghar, A. Raza, Y.F. Fan, M. Mumtaz, M. Shoaib, M. Ansar, A. Manaf, W.Y. Yang, F. Yang 2021, Crop photosynthetic response to light quality and light intensity. J Integr Agric 20:4-23. doi:10.1016/S2095-3119(20)63227-0 S2095-3119(20)63227-0

10.1016/S2095-3119(20)63227-0
21

Suh K.H. 2017, Effect of a sudden increase in light intensity on normalized difference vegetation index (NDVI) reflected from leaves of tobacco. J Environ Sci Int 26:543-547. doi:10.5322/JESI.2017.26.4.543

10.5322/JESI.2017.26.4.543
22

Wang J., W. Lu, Y. Tong, and Q. Yang 2016, Leaf morphology, photosynthetic performance, chlorophyll fluorescence, stomatal development of lettuce (Lactuca sativa L.) exposed to different ratios of red light to blue light. Front Plant Sci 7:250. doi:10.3389/fpls.2016.00250

10.3389/fpls.2016.0025027014285PMC4785143
23

Wang X.Q., Z.L. Zeng, Y.Y. Li, and W. Huang 2024, Photoinhibition and photosynthetic regulation in fluctuating light under compound stresses of drought and heat. Physiol Plant 176:e14406. doi:10.1111/ppl.14406

10.1111/ppl.14406
24

Yamori W., K. Kusumi, K. Iba, and I. Terashima 2020, Increased stomatal conductance induces rapid changes to photosynthetic rate in response to naturally fluctuating light conditions in rice. Plant Cell Environ 43:1230-1240. doi:10.1111/pce.13725

10.1111/pce.13725
25

Yang F., Y. Fan, X. Wu, Y. Cheng, Q. Liu, L. Feng, J. Chen, Z. Wang, X. Wang, T. Yong, W. Liu, J. Liu, J. Du, K. shu, W. Yang 2018, Auxin-to-gibberellin ratio as a signal for light intensity and quality in regulating soybean growth and matter partitioning. Front Plant Sci 9:56. doi:10.3389/fpls.2018.00056

10.3389/fpls.2018.0005629441084PMC5797538
26

Yin Z.H., and G.N. Johnson 2000, Photosynthetic acclimation of higher plants to growth in fluctuating light environments. Photosynth Res 63:97-107. doi:10.1023/A:1006303611365

10.1023/A:1006303611365
27

Yudina L., E. Sukhova, E. Gromova, M. Mudrilov, Y. Zolin, A. Popova, V. Nerush, A. Pecherina, A.A. Grishin, A.A. Dorokhov, V. Sukhov 2023, Effect of duration of LED lighting on growth, photosynthesis and respiration in lettuce. Plants 12:442. doi:10.3390/plants12030442

10.3390/plants1203044236771527PMC9921278
28

Zhou J., P. Li, J. Wang, and W. Fu 2019, Growth, photosynthesis, and nutrient uptake at different light intensities and temperatures in lettuce. HortSci 54:1925-1933. doi:10.21273/HORTSCI14161-19 HORTSCI14161-19

10.21273/HORTSCI14161-19
Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Journal of Bio-Environment Control
  • Journal Title(Ko) :생물환경조절학회지
  • Volume : 34
  • No :3
  • Pages :325-334
  • Received Date : 2025-06-10
  • Revised Date : 2025-07-21
  • Accepted Date : 2025-07-25
  • DOI :https://doi.org/10.12791/KSBEC.2025.34.3.325
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