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2024 Vol.33, Issue 4 Preview Page

Original Articles

Determining Light Conditions for Improved Carrot Seed Germination in a Closed-type Plant Factory

완전제어형 식물공장에서 당근 발아를 위한 광질 조건 결정

Bohyun Sung1
,
Young-Yeol Cho23*
성 보현1
,
조 영열23*
1Graduate Student, Department of Horticultural Science, Jeju National University, Jeju 63243, Korea
2Professor, Department of Horticultural Science, Jeju National University, Jeju 63243, Korea
3Researcher, Research Institute for Subtropical Agriculture and Animal Biotechnology, SARI, Jeju National University, Jeju 63243, Korea
1제주대학교 원예학과 대학원생
2제주대학교 원예학과 교수
3제주대학교 아열대농업생명과학연구소 친환경연구소 연구원
*Corresponding Author
31 October 2024. pp. 480-490
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Abstract

Germination is the first stage of the plant life cycle and affects the overall growth of the plant. The higher the germination rate, the higher the overall growth potential of the crops. This study aimed to determine the light conditions for carrot germination stages. To determine the light conditions suitable for germination, five different light conditions (R:G:B = 10:0:0, 8:0:2, 7:0:3, 6:1:3, and 0:0:10) and a dark condition were set. Germination characteristics were investigated using indices such as percent of germination (PG), germination energy (GE), mean germination time (MGT), germination rate (GR), and mean daily germination (MDG). The R:G:B = 0:0:10 treatment showed a rapid increase in the PG, exceeding 80% on the Day 5 and maintaining over 90% germination after Day 10. The R:G:B = 6:1:3 treatment showed the lowest PG in the early stages of germination, reaching 41% on the Day 5 and finally recording a germination rate of about 60%. Similarly, the R:G:B = 0:0:10 treatment showed the most favorable results in all indices: GE, MGT, GR and MDG. On the other hand, the R:G:B = 6:1:3 treatment showed the lowest performance in these indices. Therefore, it was concluded that the R:G:B = 0:0:10 treatment is suitable for germination of carrot cultivation in closed-type plant factory.

Keywords
Daucus carota L.
germination rate
light quality
percent of germination
RGB ratio

발아는 식물 생애 주기의 첫 번째 단계이며 식물의 전반적인 성장에 영향을 미친다. 발아율이 높을수록 작물의 전반적인 성장 잠재력이 높아진다. 본 연구는 당근 발아 생장 단계에 대한 최적의 광조건을 결정하고자 하였다. 발아에 적합한 광조건을 결정하기 위해 다섯 가지 다른 광조건(R:G:B= 10:0:0, 8:0:2, 7:0:3, 6:1:3 및 0:0:10)과 암조건을 설정하였다. 발아 특성은 발아율(PG), 발아 에너지(GE), 평균 발아 시간(MGT), 발아 속도(GR), 평균 일일 발아(MDG)와 같은 지표를 사용하여 조사되었다. R:G:B= 0:0:10 처리는 PG의 급격한 증가를 보여 5일째에 80%를 초과하며 10일 이후에도 90% 이상의 발아율을 유지하였다. R:G:B= 6:1:3 처리는 발아 초기 단계에서 가장 낮은 PG를 보였고, 5일째에 41%에 도달하고 최종적으로 약 60%의 발아율을 기록하였다. 유사하게, R:G:B= 0:0:10 처리는 GE, MGT, GR 및 MDG와 같은 모든 지표에서 가장 유리한 결과를 나타내었다. 반면, R:G:B= 6:1:3 처리는 이러한 지표에서 가장 낮은 성과를 보였다. 따라서 완전제어형 식물공장에서 당근 재배의 발아에는 R:G:B= 0:0:10 처리가 적합하다는 결론을 얻었다.

키워드
광질
발아속도
발아율
Daucus carota L.
RGB 비율
References
1

Ahmad M., and A.R. Cashmore 1993, HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature 366:162-166. doi:10.1038/366162a0

10.1038/366162a08232555
2

Ahmad M., and A.R. Cashmore 1996, Seeing blue: The discovery of cryptochrome. Plant Mol Biol 30:851-861. doi:10.1007/BF00020798

10.1007/BF000207988639745
3

Arscott S.A., and S.A. Tanumihardjo 2010, Carrots of many colors provide basic nutrition and bioavailable phytochemicals acting as a functional food. Compr Rev Food Sci Food Saf 9:223-239. doi:10.1111/j.1541-4337.2009.00103.x

10.1111/j.1541-4337.2009.00103.x
4

Bewley J.D., and M. Black 1994, Seeds: Physiology of development and germination. Springer, Boston, MA, USA. pp 1-33. doi:10.1007/978-1-4899-1002-8_1

10.1007/978-1-4899-1002-8_1
5

Bouly J.P., E. Schleicher, M. Dionisio-Sese, F. Vandenbussche, D.V.D. Straeten, N. Bakrim, S. Meier, A. Batschauer, P. Galland, R. Bittl, and M. Ahmad 2007, Cryptochrome blue-light photoreceptors are activated through interconversion of flavin redox states. J Biol Chem 282:9383-9391. doi:10.1074/jbc.M609842200

10.1074/jbc.M60984220017237227
6

Bradford K.J. 2002, Applications of hydrothermal time to quantifying and modeling seed germination and dormancy. Weed Sci 50:248-260. doi:10.1614/0043-1745

10.1614/0043-1745(2002)050[0248:AOHTTQ]2.0.CO;2
7

Chae J.C., S.Z. Park, B.H. Kang, and S.H. Kim 2006, Principles of cultivation (3rd). Hyang Mun Sa, Seoul, Korea. pp 294-295.

8

Cocco E., D. Farci, P. Haniewicz, W.P. Schröder, A. Maxia, and D. Piano 2022, The influence of blue and red light on seed development and dormancy in Nicotiana tabacum L. Seeds 1:152-163. doi:10.3390/seeds1030014

10.3390/seeds1030014
9

Djavanshir K., and H. Pourbeik 1976, Germination value - a new formula. Silvae Genet 25:79-83.

10.1109/TIM.1976.6312310
10

Domin M., F. Kluza, D. Góral, S. Nazarewicz, K. Kozłowicz, M. Szmigielski, and B. Ślaska-Grzywna 2020, Germination energy and capacity of maize seeds following low-temperature short storage. Sustainability 12:46. doi:10.3390/su12010046

10.3390/su12010046
11

Drewnowski A. 2010, The cost of US foods as related to their nutritive value. Am. J Clin Nutr 92:1181-1188. doi:10.3945/ajcn.2010.29300

10.3945/ajcn.2010.2930020720258PMC2954450
12

Finch-Savage W.E., and G. Leubner-Metzger 2006, Seed dormancy and the control of germination. New Phytol 171:501-523. doi:10.1111/j.1469-8137.2006.01787.x

10.1111/j.1469-8137.2006.01787.x16866955
13

Finch-Savage W.E., and G.W. Bassel 2016, Seed vigour and crop establishment: extending performance beyond adaptation. J Exp Bot 67:567-591 doi:10.1093/jxb/erv490

10.1093/jxb/erv49026585226
14

Finkelstein R.R., S.S.L. Gampala, and C.D. Rock 2008, Abscisic acid signaling in seeds and seedlings. Plant Cell 14:S15-S45. doi:10.1105/tpc.010441

10.1105/tpc.01044112045268PMC151246
15

Folta M.K., and S.A. Maruhnich 2007, Green light: A signal to slow down or stop. J Exp Bot 58:3099-3111. doi:10.1093/jxb/erm130

10.1093/jxb/erm13017630292
16

Goggin D.E., and K.J. Seadman 2012, Blue and green are frequently seen: Responses of seeds to short- and mid-wavelength light. Seed Sci Res 22:27-35. doi:10.1017/S0960258511000444

10.1017/S0960258511000444
17

Graeber K., K. Nakabayashi, E. Miatton, G. Leubner-Metzger, and W.J. Soppe 2012, Molecular mechanisms of seed dormancy. Plant Cell Environ 35:1769-1786. doi:10.1111/j.1365-3040.2012.02542.x

10.1111/j.1365-3040.2012.02542.x22620982
18

Guo H., H. Duong, N. Ma, and C. Lin 1999, The Arabidopsis blue light receptor cryptochrome 2 is a nuclear protein regulated by a blue light-dependent post-transcriptional mechanism. Plant J 19:279-287. doi:10.1046/j.1365-313x.1999.00525.x

10.1046/j.1365-313X.1999.00525.x10476075
19

Hendricks S.B., V.K. Toole, and H.A. Borthwick 1968, Opposing actions of light in seed germination of Poa pratensis and Amaranthus arenicola. Plant Physiol 43:2023-2028.

10.1104/pp.43.12.202316657003PMC1087119
20

Hwang H.J., J.M. Lee, S.Y. Kim, G.W. Choi 2008, Seed germination in lettuce affected by light quality and plant growth regulators. J Bio-Env Con 17:51-59.

21

Kim S.I., S.I. Song, and H.S. Seo 2014, Cryptochrome 2 negatively regulates ABA-dependent seed germination in Arabidopsis. J Life Sci 1:880-884.

22

KOSIS 2023, Crop production survey. https://kosis.kr/statHtml/statHtml.do?orgId=101&tblId=DT_1ET0029&conn_path=I3 Accessed 27 October 2024

23

Lee J.M. 2013, Vegetable science crop details. Hyang Mun Sa, Seoul, Korea. pp 232-245.

24

Leivar P., and P.H. Quail 2011, PIFs: pivotal components in a cellular signaling hub. Trends Plant Sci 16:19-28. doi:10.1016/j.tplants.2010.08.003

10.1016/j.tplants.2010.08.00320833098PMC3019249
25

Lin C. 2000, Plant blue-light receptors. Trends Plant Sci 5:337-342. doi:10.1016/s1360-1385(00)01687-3

10.1016/S1360-1385(00)01687-310908878
26

Mirmazloum I., A. Kiss, É . Erdélyi, M. Ladányi, É.Z. Németh, and P. Radácsi 2020, The effect of osmopriming on seed germination and early seedling characteristics of Carum carvi L. Agriculture 10:94. doi:10.3390/agriculture10040094

10.3390/agriculture10040094
27

Neff M.M. 2012, Light-mediated seed germination: Connecting phytochrome B to gibberellic acid. Dev Cell 22:687-688. doi:10.1016/j.devcel.2012.04.003

10.1016/j.devcel.2012.04.00322516192
28

Que F., X.L. Hou, G.L. Wang, Z.S. Xu, G.F. Tan, T. Li, Y.H. Wang, A. Khadr, and A.S. Xiong 2019, Advances in research on the carrot, an important root vegetable in the Apiaceae family. Hortic Res 6:69. doi:10.1038/s41438-019-0150-6.

10.1038/s41438-019-0150-631231527PMC6544626
29

Selvakumar R., P. Kalia, and R.S. Raje 2019, Genetic analysis of nutritional traits in tropical carrot (Daucus carota L.). Genetika 51:641-660. doi:10.2298/GENSR1902641S

10.2298/GENSR1902641S
30

Seo M., A. Hanada, A. Kuwahara, A. Endo, M. Okamoto, Y. Yamauchi, H. North, A. Marion-Poll, T.P. Sun, T. Koshiba, Y. Kamiya, S. Yamaguchi, and E. Nambara 2009, Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and germination. Plant J 57:680-691. doi:10.1111/j.1365-313X.2006.02881.x

10.1111/j.1365-313X.2006.02881.x17010113
31

Shen Y., R. Khanna, C.M. Carle, and P.H. Quail 2005, Phytochrome induces rapid PIF5 phosphorylation and degradation in response to red-light activation. Plant Cell 17:2553-2570. doi:10.1104/pp.107.105601

10.1104/pp.107.10560117827270PMC2048774
32

Simlat M., P. Ślęzak, M. Moś, M. Warchoł, E. Skrzypek, and A. Ptak 2016, The effect of light quality on seed germination, seedling growth and selected biochemical properties of Stevia rebaudiana Bertoni. Sci Hortic 211:295-304. doi:10.1016/j.scienta.2016.09.009

10.1016/j.scienta.2016.09.009
33

Sousa M.P, L.F. Braga, J.F. Braga, and M.E.A. Delachiave 2008, Germination of Plantago ovata Forsk. (Plantaginaceae) seeds: temperature and photoblastism. Rev Árvore 32:51-57. doi:10.1590/S0100-67622008000100007

10.1590/S0100-67622008000100007
34

Suchánková M., Z. Kapounová, M. Dofková, J. Ruprich, J. Blahová, and I. Kouřilová 2015, Selected fruits and vegetables: comparison of nutritional value and affordability. Czech J Food Sci 33:242-246. doi:10.17221/353/2014-CJFS

10.17221/353/2014-CJFS
35

Sung B.H., N.Y. Kwak, and Y.Y. Cho 2024, Determining light conditions for improved carrot growth in a closed-type plant factory. Hortic Sci Technol 42:143-150. doi:10.7235/HORT.20240012

10.7235/HORT.20240012
36

Vieira D.C.M., F. Socolowski, and M. Takaki 2010, Seed germination and seedling emergence of the invasive exotic species, Clausena excavata. Braz J Biol 70:1015-1020. doi:10.1590/s1519-69842010000500014

10.1590/S1519-6984201000050001421180907
37

Zentella R., Z.L. Zhang, M. Park, S.G. Thomas, A. Endo, K. Murase, C.M. Fleet, Y. Jikumaru, E. Nambara, Y. Kamiya, and T.P. Sun 2007, Global analysis of DELLA direct targets in early gibberellin signaling in Arabidopsis. Plant Cell 19:3037-3057. doi:10.1105/tpc.107.054999

10.1105/tpc.107.05499917933900PMC2174696
38

Zhang T., S. Fan, Y. Xiang, S. Zhang, J. Wang, and Q. Sun 2020, Non-destructive analysis of germination percentage, germination energy and simple vigour index on wheat seeds during storage by Vis/NIR and SWIR hyperspectral imaging. Spectrochim Acta Part A 239:118488. doi:10.1016/j.saa.2020.118488

10.1016/j.saa.2020.11848832470809
Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Journal of Bio-Environment Control
  • Journal Title(Ko) :생물환경조절학회지
  • Volume : 33
  • No :4
  • Pages :480-490
  • Received Date : 2024-10-02
  • Revised Date : 2024-10-29
  • Accepted Date : 2024-10-30
  • DOI :https://doi.org/10.12791/KSBEC.2024.33.4.480
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