Effect of Relative Humidity on Physiology and Antioxidant Metabolism of Grafted Watermelon Seedlings

Hao Wei; 위 호; Abinaya Manivannan; Abinaya Manivannan; Sowbiya Muneer; Sowbiya Muneer; SooHoon Kim; 김 수훈; Liu Ya; 유 아; Ji Eun Park; 박 지은; Byoung Ryong Jeong; 정 병룡

doi:10.12791/KSBEC.2016.25.4.351

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Effect of Relative Humidity on Physiology and Antioxidant Metabolism of Grafted Watermelon Seedlings 상대습도가 수박 접목묘의 생리 및 항산화 대사에 미치는 영향

30 November 2016. pp. 351-358

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Abstract

본 연구는 수박 접목묘의 활착에 미치는 상대습도의 영향을 알아보고자 수행하였다. 2품종(‘스피드’, ‘삼복꿀’)의 수박(Citrullus vulgaris Schrad.)을 ‘RS-동장군’ 박 대목(Lagenaria siceraria Stanld.)에 접목하고, 접목묘는 주간 25°C/야간 18°C, 광주기는 1일 16시간 기준으로 몰리어다이어그램을 따라 3수준의 상대습도, 95-96%[1.1-0.8(주간) 또는 0.8-0.6(야간) g·m^-3수분부족분(VPD)], 97-98%[0.7-0.4(주간) 또는 0.5-0.3(야간) g·m^-3 VPD], 또는 99-100%[0.3-0.0(주간) 또는 0.2-0.0(야간) g·m^-3 VPD]로 유지하였다. 상대습도 처리 중 97-98% 처 리는 대목과 ‘스피드’ 접수의 생체중과 초장을 가장 크게 증가시키며 접목 2일 후에 접합부의 연결을 향상시켰 다. 그러나 ‘삼복꿀’의 경우 상대습도 99-100% 처리에서 경경과 초장이 증가하였다. 또한 상대습도 95-96%와 97-98% 처리에서 재배한 2 품종 모두 과산화수소가 덜 발생하고 산화적 스트레스의 지표인 항산화 효소의 활성 이 덜 나타났다. 따라서 이 연구의 결과는 수박묘의 접목활착에 가장 적절한 상대습도는 97-98%였다.

키워드

antioxidant enzymes

grafting

oxidative stress

reactive oxygen species

vascular connection.

산화스트레스

유관속 연결

접목

활성산소

항산화효소

This study investigated the effect of different relative humidity (RH) regimes on graft healing of grafted seedlings of watermelon (Citrullus vulgaris Schrad.). Two watermelon cultivars (‘Speed’ and ‘Sambok Honey’) were grafted onto the ‘RS-Dongjanggun’ bottle gourd rootstock (Lagenaria siceraria Stanld.) and the grafted seedlings were maintained under one of three relative humidity regimes, 95-96% [1.1-0.8 (day) or 0.8-0.6 (night) g·m^-3 vapor pressure deficit (VPD)], 97-98% [ 0.7-0.4 (day) or 0.5-0.3 (night) g·m^-3 (VPD)], or 99-100% [0.3-0.0 (day) or 0.2-0.0 (night) g·m^-3 (VPD)] according to the Mollier diagram based on the air temperature of 25°C day/18°C night with 16 h photoperiod per day. Among the RH treatments, 97-98% significantly increased plant height and fresh weight of the rootstock and scion of the ‘Speed’ and it also enhanced the graft union connection of both cultivars after two days of grafting. However, plant height and thickness of the scion of ‘Sambok Honey’ was increased by the 99-100% RH treatment. Furthermore, both cultivars grown in the 95-96 and 97-98% RH treatments consisted of lower levels of endogenous H₂O₂ and less activities of antioxidant enzymes which illustrated the occurrence of less oxidative stress. Hence, the results of this study identified the optimal RH level for the graft healing of watermelon seedlings.

키워드

antioxidant enzymes

grafting

oxidative stress

reactive oxygen species

vascular connection.

산화스트레스

유관속 연결

접목

활성산소

항산화효소

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Information

Publisher :The Korean Society for Bio-Environment Control
Publisher(Ko) :(사)한국생물환경조절학회
Journal Title :Protected Horticulture and Plant Factory
Journal Title(Ko) :시설원예ㆍ식물공장
Volume : 25
No :4
Pages :351-358
Received Date : 2016-11-30
Revised Date : 2016-12-23
Accepted Date : 2016-12-23
DOI :https://doi.org/10.12791/KSBEC.2016.25.4.351

Journal of Bio-Environment Control ISSN:1229-4675(Print) 2765-3641(Online) 생물환경조절학회지

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Literature Cited