All Issue

2023 Vol.32, Issue 4 Preview Page

Original Articles

Seed and Germination Characteristics of Allium koreanum H.J. Choi & B.U. Oh for Effective Propagation

효과적인 번식을 위한 돌부추의 종자 및 발아 특성

Hee Sung Hwang12
,
Jeong Hun Hwang3
,
Ji Hye Yun3
,
So Yeong Hwang3
,
Ji Eun Park34
,
Hyeon Eui Oh1
,
Sang Jun Lee5
,
Jeong Mi Park6
,
Seung Jae Hwang789*
황 희성12
,
황 정훈3
,
윤 지혜3
,
황 소영3
,
박 지은34
,
오 현의1
,
이 상준5
,
박 정미6
,
황 승재789*
1Graduate Student, Division of Crop Science, Graduate School of Gyeongsang National University, Jinju 52828, Korea
2Researcher, Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea
3Graduate Student, Department of Applied Life Science, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea
4Researcher, Biological Resources Utilization Division, National Institute of Biological Resources (NIBR), Miryang 50452, Korea
5Researcher, Species Diversity Research Division, National Institute of Biological Resources (NIBR), Incheon 22689, Korea
6Researcher, Biological Resources Assessment Division, National Institute of Biological Resources (NIBR), Incheon 22689, Korea
7Professor, Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea
8Professor, Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea
9Professor, Division of Horticultural Science, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea
1경상국립대학교 작물생산과학부, 대학원생
2경상국립대학교 농업생명과학연구원, 연구원
3경상국립대학교 응용생명과학부, 대학원생
4국립생물자원관 생물소재활용과, 연구원
5국립생물자원관 생물종다양성연구과, 연구원
6국립생물자원관 생물소재분석과, 연구원
7경상국립대학교 농업생명과학연구원, 교수
8경상국립대학교 생명과학연구원, 교수문소속
9경상국립대학교 원예과학부, 교수
*Corresponding Author
31 October 2023. pp. 359-365
PDF XML Citation
Abstract

Native plants are unique genetic resources that have the potential to be used as ornamental, medical, and food resources. Allium koreanum H.J. Choi & B.U. Oh is one of the native plants distributed in the coastal rocky land of Korea, which has high conservation value due to climate change and reduction of its habitat. This study was conducted to investigate the effects of temperature and hydrogen peroxide on the germination of A. koreanum. The seeds were prepared as untreated (control) or those treated with 1% hydrogen peroxide for 90 minutes (H2O2), and the treatments were placed in plant growth chambers set at 15, 20, and 25°C. Regardless of the H2O2 treatment, the germination percentage at 15°C, which was 42%, was more than two times higher than that at 20°C and 25°C, which were 18% and 0%, respectively, 23 days after sowing. The number of days to attain 50% of the final germination percentage (T50) was the shortest at 20°C, but the mean daily germination (MDG) was the highest at 15°C. Therefore, 1% of H2O2 treatment did not have a significant effect on the germination percentage of A. koreanum, and a temperature of 15°C was considered to be optimum to increase the germination percentage of A. koreanum. The results of this study can be used as basic research data for the germination of A. koreanum.

Keywords
conservation
mean daily germination
native plant
plant growth chamber
rocky land

자생식물은 관상용, 약용, 식량자원으로 활용될 수 있는 잠재력을 지닌 고유 유전자원이다. 돌부추(Allium koreanum H.J. Choi & B.U. Oh)는 우리나라 해안 암반지대에 분포하는 자생식물 중 하나로, 기후변화와 서식지 감소로 인해 보전 가치가 높은 식물이다. 이번 연구는 온도와 과산화수소가 돌부추의 발아에 미치는 영향을 조사하기 위해 수행되었다. 종자를 무처리(대조군) 또는 1% 과산화수소(H2O2)로 90분간 처리한 종자를 준비해 15, 20, 25°C로 설정된 식물 생장 챔버에 배치하였다. 그 결과, 파종 23일 후 15°C에서 42%인 발아율이 20°C와 25°C에서 각각 18%와 0%인 발아율보다 2배 이상 높았으며, H2O2 처리 여부와 관계없이 15°C에서 발아율이 42%로 가장 높았다. H2O2 처리와 관계없이 최종 발아율 50%(T50)에 도달하는 일수는 20°C에서 가장 짧았지만, 일평균 발아율(MDG)은 15°C에서 가장 높았다. 따라서 1%의 H2O2 처리는 돌부추의 발아율에 큰 영향을 미치지 않았으며, 15°C의 온도가 돌부추의 발아율을 높이는 데 최적인 것으로 판단된다. 본 연구 결과는 돌부추의 발아를 위한 기초 연구 자료로 활용될 수 있을 것으로 기대된다.

키워드
보존
평균 일일 발아
자생 식물
식물 생장 챔버
암석지
References
1
Bailly C., H. El-Maarouf-Boutau, and F. Corbineau 2008, From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology. C R Biol 331:806-814. doi:10.1016/j.crvi.2008.07.022 10.1016/j.crvi.2008.07.02218926495
2
Barba-Espín G., P. Diaz-Vivancos, M.J. Clemente-Moreno, A. Albacete, L. Faize, M. Faize, F. Pérez-alfocea, and J.A. Hernández 2010, Interaction between hydrogen peroxide and plant hormones during germination and the early growth of pea seedlings. Plant Cell Environ 33:981-994. doi:10.1111/j.1365-3040.2010.02120.x 10.1111/j.1365-3040.2010.02120.x20102539
3
Baskin J.M., and C.C. Baskin 2004, A classification system for seed dormancy. Seed Sci Res 14:1-16. doi:10.1079/SSR2003150 10.1079/SSR2003150
4
Choi H.J., and B.U. Oh 2011, A partial revision of Allium (Amaryllidaceae) in Korea and north-eastern China. Bot J Linn Soc 167:153-211. doi:10.1111/j.1095-8339.2011.01166.x 10.1111/j.1095-8339.2011.01166.x
5
Choi H.J., C.G. Jang, and B.U. Oh 2004a, A taxonomic review of Korean Allium (Alliaceae). Korean J Plant Taxon 34:119-152. (in Korean) 10.11110/kjpt.2004.34.2.119
6
Choi H.J., C.G. Jang, S.C. Ko, and B.U. Oh 2004b, Two new texa of Allium (Alliaceae) from Korea: A. koreanum H.J. Choi et B.U. Oh and A. thunbergii var. teretifolium H.J. Choi et B.U. Oh. Korean J Plant Taxon 34:75-85. doi:10.11110/kjpt.2004.34.2.075 (in Korean) 10.11110/kjpt.2004.34.2.075
7
Czech A., M. Szmigielski, and I. Sembratowicz 2022, Nutritional value and antioxidant capacity of organic and conventional vegetables of the genus Allium. Sci Rep 12:18713. doi:10.1038/s41598-022-23497-y 10.1038/s41598-022-23497-y36333512PMC9636188
8
Farooq M., S.M.A. Basra, B.A. Saleem, M. Nafees, and S.A. Chishti 2005, Enhancement of tomato seed germination and seedling vigor by osmopriming. Pak J Agric Sci 42:36-41.
9
Fenwick G.R., A.B. Hanley, and J.R. Whitaker 1985, The genus Allium-part 1. Crit Rev Food Sci Nutr 22:199-271. doi:10.1080/10408398509527415 10.1080/104083985095274153902370
10
Finch-Savage W.E., G. Leubner-Metzger 2006, Seed dormancy and the control of germination. New Phytol 171:501-523. doi:10.1111/j.1469-8137.2006.01787.x 10.1111/j.1469-8137.2006.01787.x16866955
11
Gairola K.C., A.R. Nautiyal, and A.K. Dwivedi 2011, Effect of temperatures and germination media on seed germination of Jatropha curcas Linn. Adv Bioresour 2:66-71.
12
Gardner F.P., R.B. Pearce, and R.L. Mitchell 2020, Seed and germination. Physiology of crop plants, Translated by Nam SY, Ed 1, RBG Press, Seoul, Korea, pp 241-277. (in Korean)
13
Geneve R.L. 2003, Impact of temperature on seed dormancy. HortScience 38:336-340. 10.21273/HORTSCI.38.3.336
14
Gutterman Y., R. Kamenetsky, and M. van Rooyen 1995, A comparative study of seed germination of two Allium species from different habitats in the Negev desert highlands. J Arid Environ 29:305-315. doi:10.1016/S0140-1963(05)80109-0 10.1016/S0140-1963(05)80109-0
15
Han J.W., E.H. Kim, M.W. Oh, J.H. Lee, K.H. Ma, Y.H. Yoon, and J.T. Jeong 2022, Seed morphology and germination characteristics of Rehmannia glutinosa. Korean J Med Crop Sci 30:187-194. doi:10.7783/KJMCS.2022.30.3.187 10.7783/KJMCS.2022.30.3.187
16
Imani A., M. Rasouli, R. Tavakoli, E. Zarifi, R. Fatahi, G. Barba-Espin, and P. Martinez-gomez 2011, Optimization of seed germination in Prunus species combining hydrogen peroxide or gibberellic acid pre-treatment with stratification. Seed Sci Technol 39:204-207. doi:10.15258/sst.2011.39.1.18 10.15258/sst.2011.39.1.18
17
Kim J.M., C.H. Park, S.Y. Kim, Y.J. Lim, and J.Y. Jung 2021, Seed germination responses to different incubation temperatures and light treatments in for Allium species native to Korea. Flower Res J 29:173-180. doi:10.11623/frj.2021.29.3.07 10.11623/frj.2021.29.3.07
18
KMA 2023, Temperature analysis. Available via https://data.kma.go.kr/stcs/grnd/grndTaList.do?pgmNo=70 Accessed 08 February 2023
19
Korea Forest Service 2008, National biological information system. Available via http://www.nature.go.kr/kbi/plant/pilbk/selectPlantPilbkDtl.do?plantPilbkNo=41345#dtlInfo Accessed 07 December 2022
20
Lariguet P., P. Ranocha, M. De Meyer, O. Barbier, C. Penel, and C. Dunand 2013, Identification of a hydrogen peroxide signaling pathway in the control of light-dependent germination in Arabidopsis. Planta 238:381-395. doi:10.1007/s00425-013-1901-5 10.1007/s00425-013-1901-523716184
21
Lee J.Y., and Y.Y. Lee 2014, Plant germplasm preservation research system of national center for genetic resources preservation (NCGRP) in United State. J Korean Soc for Seed Sci Ind 11:10-17.
22
Martin A.C. 1946, The comparative internal morphology of seeds. Am Midl Nat 36:513-660. doi:10.2307/2421457 10.2307/2421457
23
Na Y.W., Y.M. Choi, H.J. Beak, S.Y. Lee, J.H. Kang, and S.H. Kim 2014, Seed longevity of rice germplasm in the national agrobiodiversity center. Korean J Crop Sci 59:216-222. doi:10.7740/kjcs.2014.59.3.216 (in Korean) 10.7740/kjcs.2014.59.3.216
24
Nadimi M., G. Loewen, P. Bhowmik, and J. Paliwal 2022, Effect of laser biostimulation on germination of sub-optimally stored flaxseeds (Linum usitatissimum). Sustainability 14:12183. doi:10.3390/su141912183 10.3390/su141912183
25
National Biodiversity Center 2018, National biodiversity information sharing system. Available via https://kbr.go.kr/home/rsc/rsc01002v.do?data_gbn_cd=BIO&ktsn_no=120000065212&menuKey=448 Accessed 08 December 2022
26
Naturing 2019, Nature observation. Available via https://www.naturing.net/o/339481 Accessed 08 December 2022
27
NIBR 2023, National institute of biological resources biodiversity of the Korean peninsula. Available via https://species.nibr.go.kr/species/speciesDetail.do?ktsn=120000065212&tab=#; Accessed 08 February 2023
28
Nongsaro 2008, Spinach seed hydrogen peroxide treatment. Available via https://www.nongsaro.go.kr/portal/ps/psz/psza/contentSub.ps;jsessionid=vxJiA9Fl7wDuha5zllk2DH5OuenjwDFFWL6UksOzct7txU4900MFXrcIF1Na19Vw.nongsaro-web_servlet_engine1?menuId=PS00098&pageIndex=1&pageSize=10&sYear=1998&sNationCode=%ED%98%B8%EC%A3%BC&valAll=&sType=sCntntsSjorDtl&cntntsNo=27444&sKidofcomdtySeCode=VC010902&sText= Accessed 01 July 2022
29
Ogawa K., and M. Iwabuchi 2001, A mechanism for promoting the germination of Zinnia elegans seeds by hydrogen peroxide. Plant Cell Physiol 42:286-291. doi:10.1093/pcp/pce032 10.1093/pcp/pce03211266579
30
Park H.J., and B.G. Jung 2017, Optimal contract under the Nagoya protocol for the benefit sharing. Environ Resour Econ Rev 26:85-101. doi:10.15266/KEREA.2017.26.1.085
31
Rajametov S., Y.Y. Lee, Y.C. Kim, S.Y. Lee, J.Y. Yi, Y.A. Jeon, J.S. Sung, G.A. Lee, and J.G. Gwak 2014, Response of pre and post treatments for cryopreservation of Korean ginseng seeds on recovering viability. Korean J Breed Sci 46:408-416. doi:10.9787/KJBS.2014.46.4.408 10.9787/KJBS.2014.46.4.408
32
Sharifi-Rad J., D. Mnayer, G. Tabanelli, Z.Z. Stojanovic-Radic, M. Sharifi-Rad, Z. Yousaf, L. Vallone, W.N. Setzer, and M. Iriti 2016, Plants of the genus Allium as antibacterial agents: from tradition to pharmacy. Cell Mol Biol 62:57-68.
33
Taran M., M. Rezaeian, and M. Izaddoost 2006, In vitro antitrichomonas activity of Allium hirtifolium (Persian shallot) in comparison with metronidazole. Iran J Publ Health 35:92-94.
34
Thompson K., J.P. Grime, and G. Mason 1977, Seed germination in response to diurnal fluctuation of temperature. Nature 267:147-149. doi:10.1038/267147a0 10.1038/267147a016073423
35
Wojtyla Ł., K. Lechowska, S. Kubala, and M. Garnczarska 2016, Different modes of hydrogen peroxide action during seed germination. Front Plant Sci 7:66. doi:10.3389/fpls.2016.00066 10.3389/fpls.2016.0006626870076PMC4740362
36
Xiao H., H. Yang, T. Monaco, Q. Song, and Y. Rong 2020, Modeling the influence of temperature and water potential on seed germination of Allium tenuissimum L. PeerJ 8:e8866. doi:10.7717/peerj.8866 10.7717/peerj.886632292650PMC7147430
37
Yoshioka T., T. Endo, and S. Satoh 1998, Restoration of seed germination at supraoptimal temperatures by fluridone, an inhibitor of abscisic acid biosynthesis. Plant Cell Physiol 39:307-312. doi:10.1093/oxfordjournals.pcp.a029371 10.1093/oxfordjournals.pcp.a029371
Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Journal of Bio-Environment Control
  • Journal Title(Ko) :생물환경조절학회지
  • Volume : 32
  • No :4
  • Pages :359-365
  • Received Date : 2023-09-05
  • Revised Date : 2023-10-23
  • Accepted Date : 2023-10-24
  • DOI :https://doi.org/10.12791/KSBEC.2023.32.4.359
Journal Informaiton Journal of Bio-Environment Control Journal of Bio-Environment Control
Online Submission
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crossref funder-registry
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close