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2022 Vol.31, Issue 3 Preview Page

Original Articles

Changes in Abscisic Acid, Carbohydrate, and Glucosinolate Metabolites in Kimchi Cabbage Treated with Glutamic Acid Foliar Application under Extremely Low Temperature Conditions

이상저온 시 글루탐산 엽면 처리에 의한 배추의 ABA, 탄수화물 및 Glucosinolate 대사체 변화

Ha Seon Sim1†
,
Jung Su Jo2†
,
Ui Jeong Woo1
,
Yu Hyun Moon1
,
Tae Yeon Lee1
,
Hee Ju Lee3
,
Seung Hwan Wi3
,
Sung Kyeom Kim4*
심 하선1†
,
조 정수2†
,
우 의정1
,
문 유현1
,
이 태연1
,
이 희주3
,
위 승환3
,
김 성겸4*
1Graduate Student, Department of Horticultural Science, College of Agricultural and Science, Kyungpook National University, Daegu 41566, Korea
2Research Professor, Institute of Agricultural Science and Technology, Kyungpook National University, Daegu 41566, Korea
3Research Scientist, Vegetable Research Division, National Institute of Horticultural and Herbal Science, Wanju 55365, Korea
4Professor, Department of Horticultural Science, College of Agricultural and Science, Kyungpook National University, Daegu 41566, Korea
1경북대학교 대학원 원예과학과 대학원생
2경북대학교 농업과학기술연구소 연구초빙교수
3국립원예특작과학원 채소과 농업연구사
4경북대학교 원예과학과 교수
*Corresponding Author
†These authors contributed equally to this work.
31 July 2022. pp. 170-179
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Abstract

Glutamic acid is a precursor of essential amino acids that play an important role in plant growth and development. It is one of the biostimulants that reduce cold stress damage by stimulating biosynthetic pathways leading to cryoprotectants. This study evaluated the effects of glutamic acid foliar application on Kimchi cabbage under low-temperature stress. There were six treatments, combining three photo-/dark periods temperature levels (11/-1°C extremely low, E; 16/4°C moderately low, M; and 21/9°C optimal, O) with and without glutamic acid foliar application (0 and 10 mg·L-1; Glu 0 and Glu 10). Glutamic acid foliar application was sprayed once 10 days after transplanting, and then temperature treatment immediately after glutamic acid foliar application was conducted for up to four days. After four days of treatment, abscisic acid (ABA), phaseic acid (PA), dihydrophaseic acid (DPA), and abscisic acid-glucose ester (ABA-GE) contents were higher with Glu 10 treatment than Glu 0 treatment in M ​​treatment. Glucose content was highest in E with Glu 10 treatment (52.1 mg·100 g-1 dry weight), while fructose content was highest in O with Glu 0 treatment (134.6 mg·100 g-1 dry weight). The contents of glucolepiddin (GLP), glucobrassicin (GBS), 4-methoxyglucobrassicin (4MGBS), neoglucobrassicin (GNBS), and gluconasturtiin (GNS) were highest among all treatments in E with Glu 10 treatments (0.72, 2.05, 1.67, 9.40 and 0.85 µmol·g-1 dry weight). After two days of treatment, rapid changes in PA and DPA contents of E with Glu 10 treatments were confirmed, and several individual glucosinolate contents (GLP, GBS, 4MGBS, GNBS, and GNS) were significantly different depending on low temperature and glutamic acid treatment. In addition, the content of fructose was significantly lower than that of O treatment in E and M treatments after four days of treatment. Therefore, although the changes in PA, DPA, glucose, fructose, and individual glucosinolates according to low temperature and glutamic acid foliar treatment were shown. A clear correlation between low temperature and glutamic acid effects could not be evaluated. Results indicated that Brassica crops are cryophilic vegetables, do not react sensitively to low temperatures, and mostly have cold resistance.

Keywords
ABA metabolite, biostimulant
Brassica rapa L. subsp. pekinensis (Lour.) Hanelt
low temperature stress
phytochemical

글루탐산은 식물의 생장과 발달에 중요한 역할을 하는 필수 아미노산의 전구체이며, 저온 보호 물질로 이어지는 생합성 경로를 자극하여 저온 피해를 줄이는 생물자극제 중 하나이다. 본 연구에서는 저온 스트레스 조건에서 글루탐산 엽면 처리가 배추에 미치는 영향을 평가하였다. 글루탐산 2가지 엽면 시비 농도(0 및 10mg·L-1)와 3가지 주/야간 온도 수준(11/－1 °C extremely low, E; 16/4°C moderately low, M; 21/9°C optimal, O)을 결합하여 6개의 처리가 수행되었다. 글루탐산의 엽면 처리는 정식 후 10일에 1회 살포하고, 글루탐산 처리 직후 온도 처리는 최대 4일 동안 실시하였다. 처리 4일 후, ABA, PA, DPA 및 ABA-GE 함량은 M 처리에서 Glu 0 처리보다 Glu 10 처리에서 함량이 더 높았다. Glucose 함량은 E 및 Glu 10 처리에서 가장 높은 반면(52.1mg·100g-1 dry weight), fructose 함량은 O 및 Glu 0 처리에서 함량이 가장 높았다(134.6mg·100g-1 dry weight). GLP, GBS, 4MGBS, GNBS 및 GNS 함량은 E 및 Glu 10 처리에서 모든 처리 중 가장 높았다(0.72, 2.05, 1.67, 9.40 및 0.85µmol·g-1 dry weight). 처리 2일 후 E 및 Glu 10 처리의 PA와 DPA함량에서 급격한 변화를 확인하였고, 몇몇 개별 glucosinolate 함량(GLP, GBS, 4MGBS, GNBS 및 GNS)은 저온과 글루탐산 처리에 따라 유의적 차이를 확인할 수 있었다. 또한, fructose는 glucose 대신 fructan을 에너지원으로 사용하였기 때문에 처리 4일후 E와 M처리에서 O 처리에 비하여 유의적으로 낮은 함량을 보였다. 따라서, 저온과 글루탐산 엽면 처리에 따른 PA, DPA, glucose, fructose 및 개별 glucosinolate 물질들의 변이를 확인하였지만, 저온과 글루탐산의 효과에 관한 명확한 상관관계를 평가할 수는 없었다. 배추과 작물은 호냉성 채소로서 저온에 민감하게 반응하지 않고, 대부분 내한성을 가지고 있기 때문으로 판단된다.

키워드
ABA 대사체
Brassica rapa L. subsp. pekinensis(Lour.) Hanelt
생물자극제
저온 스트레스
파이토케미컬
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Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Journal of Bio-Environment Control
  • Journal Title(Ko) :생물환경조절학회지
  • Volume : 31
  • No :3
  • Pages :170-179
  • Received Date : 2022-06-17
  • Revised Date : 2022-07-12
  • Accepted Date : 2022-07-13
  • DOI :https://doi.org/10.12791/KSBEC.2022.31.3.170
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