Effect of Low-Pressure Mercury UV-C Lamps on the Inactivation of Agrobacterium rhizogenes in Raw Water for Facility Cultivation

Jung-Hoo Kook; Sang-Min Kim; Oluwasegun Moses Ogundele; Jeong-In Choi; Jinwoo Kim; Hyeon-Tae Kim

doi:10.12791/KSBEC.2025.34.3.298

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2025 Vol.34, Issue 3 Preview Page Next Page

Original Articles

Effect of Low-Pressure Mercury UV-C Lamps on the Inactivation of Agrobacterium rhizogenes in Raw Water for Facility Cultivation 저압 수은 UV-C 램프가 시설재배용 원수 내 Agrobacterium rhizogenes 살균에 미치는 영향

31 July 2025. pp. 298-307

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Abstract

This study quantitatively evaluated the disinfection performance of a low-pressure mercury UV-C system against the pathogenic bacterium Agrobacterium rhizogenes by analyzing the effect of different combinations of lamp power consumption and flow rate. Agricultural water samples were collected from three commercial farms (Sources A, B, and C) in Sacheon-si, Gyeongsangnam-do, for the first experiment, and Source D in Gunbuk-myeon, Haman-gun, Republic of Korea, for the second experiment. All water samples were artificially inoculated with A. rhizogenes prior to treatment. The experiment tested nine combinations of three lamp power consumption levels (40, 75, and 105 W) and three flow rates (10, 15, and 30 L/min). Among these, samples #10, #20, and #30 were inoculated but not UV-treated, serving as the corresponding pre-disinfection controls. In the second experiment, a fixed power of 75 W was applied under high-flow conditions (60 and 120 L/min), with samples #37 and #38 serving as the corresponding untreated controls. In all tested conditions, absence of detectable bacteria was confirmed, except for sample #33 (75 W, 60 L/min), in which viable cells were detected at 2.0×10² CFU/mL. However, this represented approximately a 4.9 log reduction compared to its control (#37, 1.6×10⁷ CFU/mL), indicating substantial bactericidal efficacy. These findings suggest that effective disinfection can be achieved even under high-flow conditions, provided that sufficient UV dose is delivered. The results underscore the practical applicability of low-pressure UV-C systems in agricultural water treatment through the optimal configuration of operating parameters.

Keywords

agricultural water

fisher’s exact test

flow rate

UV-C lamp power consumption

본 연구는 병원성 박테리아 Agrobacterium rhizogenes에 대해 저압 수은 UV-C 소독 시스템의 살균 성능을 정량적으로 평가하고, 램프 소비전력과 유량의 조합에 따른 소독 효율에 미치는 영향을 분석하였다. 농업용수는 경상남도 사천시 소재 세 농가(A, B, C)와 함안군 군북면의 한 농가(D)에서 채취하였으며, 목표 병원균을 인위적으로 접종한 뒤 실험을 수행하였다. 실험은 1차와 2차로 구분되며, 1차 실험은 소비전력 40, 75, 105W와 유량 10, 15, 30L/min의 3×3 조합으로 구성되었고, 2차 실험은 소비전력 75W 조건 하에서 고유량(60, 120L/min) 조건을 적용하였다. 대부분의 실험 조건에서 박테리아가 완전히 사멸되었으며, 유일하게 생균이 검출된 조건(#33, 75W－60L/min)에서만 생균이 검출되었다. 그러나 해당 조건에서도 생균수는 UV 비처리 대조군(#37)에 비해 약 4.9log 수준 감소하여 유의미한 소독 효과가 확인되었다. 이러한 결과는 고유량 조건에서도 충분한 UV 조사량이 확보된다면 안정적인 소독이 가능함을 시사한다. 본 연구는 저압 UV-C 시스템의 운전 변수에 따라 살균 효율이 어떻게 달라지는지를 실험적으로 입증하였으며, 농업용수 처리에 있어 해당 기술의 실용적 적용 가능성을 제시하였다.

키워드

농업용수

소비전력

유량

피셔의 정확 검정

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Information

Publisher :The Korean Society for Bio-Environment Control
Publisher(Ko) :(사)한국생물환경조절학회
Journal Title :Journal of Bio-Environment Control
Journal Title(Ko) :생물환경조절학회지
Volume : 34
No :3
Pages :298-307
Received Date : 2025-05-28
Revised Date : 2025-07-14
Accepted Date : 2025-07-22
DOI :https://doi.org/10.12791/KSBEC.2025.34.3.298

Journal of Bio-Environment Control ISSN:1229-4675(Print) 2765-3641(Online) 생물환경조절학회지

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