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2018 Vol.27, Issue 3 Preview Page
Chlorophyll Fluorescence, Chlorophyll Content, Graft-taking, and Growth of Grafted Cucumber Seedlings Affected by Photosynthetic Photon Flux of LED Lamps

LED 램프의 광합성유효광양자속이 오이접목묘의 엽록소형광, 엽록소함량, 활착 및 생장에 미치는 영향

Hyeong Gon Kim1
,
Jae Su Lee23
,
Yong Hyeon Kim14*
김 형곤1
,
이 재수23
,
김 용현14*
1Institute for Agricultural Machinery & ICT Convergence, Chonbuk National University, Jeonju 54896, Korea
2Farming Automation Division, Dept. of Agricultural Engineering, National Institute of Agricultural Sciences, RDA, Wanju-gun, 55365, Korea
3Dept. of Bioindustrial Precision Machinery Engineering, Graduate School, Chonbuk National University, Jeonju 54896, Korea
4Dept. of Bioindustrial Machinery Engineering, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea
1전북대학교 농업기계ICT융합연구소
2국립농업과학원 농업공학부 스마트팜개발과
3전북대학교 대학원 생물산업정밀기계공학과
4전북대학교 농업생명과학대학 생물산업기계공학과
*Corresponding author: yhkim@jbnu.ac.kr
30 July 2018. pp. 231-238
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Abstract

Chlorophyll fluorescence, chlorophyll content, graft-taking and growth of grafted cucumber seedlings as affected by photosynthetic photon flux (PPF) of LED lamps were analyzed in this study. Four PPF levels, namely 25, 50, 100, 150μmol·m-2·s-1 were provided to investigate the effect of light intensity on the chlorophyll fluorescence, chlorophyll content, graft-taking and growth of grafted cucumber seedlings. Air temperature, relative humidity, and photoperiod for graft-taking were maintained at 25°C, 90%, 16h·d-1, respectively. Maximum quantum yield (Fv/Fm) of rootstock as affected by PPF was found to be 0.84-0.85 and there was no significant change in Fv/Fm. Even though Fv/Fm of scion measured at 2 days after grafting was lowered to 0.81-0.82, after then it gradually increased with increasing PPF. At 4 days after grafting, the chlorophyll content extracted from scion increased with increasing PPF. Graft-taking ratio of grafted cucumber seedlings was 90-95% as PPF was ranged from 25μmol·m-2·s-1 to 100μmol·m-2·s-1. However, the graft-taking ratio of grafted seedlings healed under PPF of 150μmol·m-2·s-1 was decreased to 80%. Maximum PPF measured required for smooth joining of rootstock and scion was assumed to be 100μmol·m-2·s-1. At healing stage of grafted cucumber seedlings, Fv/Fm of scion decreased and at least two days after grafting were required for rooting of grafted seedlings. Chlorophyll fluorescence response of rootstock and scion was linked to light irradiation. Therefore, it was concluded that physical environment including light and humidity during healing process of grafted seedlings should be controlled more precisely to facilitate root formation and to prevent scion from lowering Fv/Fm. Further studies are required to investigate the effects of root development and joining of vascular bundles of grafted seedlings on the chlorophyll content of scion.

Keywords
chlorophyll
cucumber seedlings
grafting
maximum quantum yield
photosynthetic photon flux

본 연구는 대목과 접수의 결합 단계에서 나타나는 스 트레스를 오이접목묘의 엽록소형광반응, 엽록소함량, 활 착 및 생장 특성 측면에서 분석하고자 수행되었다. 이를 위해서 활착실 내의 광합성유효광양자속은 25, 50, 100, 150μmol· m-2·s-1의 4수준으로 설정되었고, 기온, 상대습도 및 LED 램프의 광주기는 각각 25oC, 90%, 16h·d-1이었 다. 본 연구에서 얻어진 결과를 요약하면 다음과 같다. 대목의 최대양자수율은 0.84-0.85로서 광량에 따른 분명 한 차이가 나타나지 않았다. 한편, 접수의 최대양자수율 은 접목 후 2일째에 0.81-0.82로 낮게 나타났으나, 3일 째부터 광량이 높을수록 접수의 최대양자수율이 증가하 였다. 활착 후 4일째에 측정된 접수의 엽록소함량은 광 량이 증가할수록 높게 나타났다. 오이접목묘의 활착율은 광량이 100μmol· m-2·s-1 이하일 때 90-95% 정도로 높게 나타났으나, 150μmol· m-2·s-1의 처리구에서는 80% 정도 로 저하되었다. 광주기에 따라 다르나, 오이접목묘의 활 착에 적합한 한계 광량은 플러그 트레이 표면에 조사된 광량을 기준으로 100μmol· m-2·s-1 정도이다. 본 연구에서 처리된 광강도 하에서 활착된 오이접목묘의 발근에 최소 2일이 소요되었고, 이 기간에 접수의 최대양자수율은 최 저치로 나타났다. 활착 단계에서 조사되는 광량에 따라 대목과 접수의 변이형광과 최대양자수율이 다르게 나타 났다. 그러므로 접목묘의 활착 단계에서 나타나는 스트 레스를 줄이면서 대목의 발근을 촉진하고, 접수의 최대 양자수율이 급격하게 저하되는 것을 방지하려면 광 및 습도 등의 물리적 환경이 정확하게 제어되어야 한다. 향 후 접목묘의 활착 단계에서 대목의 발근, 통도조직의 결 합 상태, 수분의 이동에 따른 엽록소함량 변화를 정량적 으로 구명할 필요가 있다.

키워드
광합성유효광양자속
엽록소
오이묘
접목
최대양자수율
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Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Protected Horticulture and Plant Factory
  • Journal Title(Ko) :시설원예ㆍ식물공장
  • Volume : 27
  • No :3
  • Pages :231-238
  • Received Date : 2018-05-16
  • Revised Date : 2018-07-04
  • Accepted Date : 2018-07-05
  • DOI :https://doi.org/10.12791/KSBEC.2018.27.3.231
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