Changes of Plant Growth,  Leaf Morphology and Cell Elongation of Spinacia oleracea Grown under Different Light-Emitting Diodes

Myungok Lee; Sangmin Park; Eunkyung Cho; Jinhee An; Eunyoung Choi

doi:10.12791/KSBEC.2018.27.3.222

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2018 Vol.27, Issue 3 Preview Page Next Page

Changes of Plant Growth, Leaf Morphology and Cell Elongation of Spinacia oleracea Grown under Different Light-Emitting Diodes 발광다이오드 광원에 따른 시금치 생육, 엽 형태형성 및 세포길이 변화

30 July 2018. pp. 222-230

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Abstract

This study aimed to determine effects of light-emitting diodes on plant growth, leaf morphology and cell elongation of two cultivars (‘World-star’ and ‘Sushiro’) of Spinacia oleracea. Plants were grown in a NFT system for 25 days after transplanting (DAT) under the LEDs [White (W), Red and Blue (RB, ratio 2:1), Blue (B), Red (R) LED] under the same light intensity and photoperiod (130 μmol·m^-2·s^-1, 12 hours). The ‘World-star’ variety was significantly higher in shoot fresh and dry weights, leaf number, and leaf area than the ‘Sushiro’ variety. For the ‘Worldstar’ variety, the two treatments of mixed light (RB) and red light (R) showed a 35% higher shoot dry weight than that of blue light (B) and white light (W) at 25 DAT. In the ‘Sushiro’ variety, mixed light (RB) treatment, which had the highest shoot fresh and dry weights, showed 40% higher than the white light (W) treatment, which had the lowest shoot fresh and dry weights. Both varieties showed leaf epinasty symptom at 21 DAT only in both mixed light (RB) and red light (R), and red light (R) treatment showed significantly higher symptom than mixed light (RB), indicating the leaf epinasty is associated with red light. Microscopic observations of the cell size in the leaf center and edge parts showed that the cell density of leaf edge under the red light (R) was lower than that in leaf center, supporting previous reports that suggest an association of the cell size difference between the leaf center and edge with the leaf epinasty occurrence. Since the blue light (B) plays a role in alleviating the epinasty symptom caused by the red light (R), it seems necessary to identify the appropriate mixing ratio of the two light sources. In addition, the Worldstar variety seems to be more suitable for the cultivation of plant factory using LED light sources.

Keywords

photomorphogenic response

leaf epinasty index

photosynthesis rate

specific leaf weight

본 실험은 LED 광원이 시금치 품종 별 생육, 잎 형태 변화 및 세포 길이에 대한 영향을 구명하고자 수행하였 다. 최아된 시금치(Spinacia oleracea.) 품종 ‘월드스타’ 와 ‘수시로’를 버미큘라이트에 육묘한 후 NFT 시스템에 정식 한 뒤 LED 적색광(R), 청색광(B), 혼합광(적색:청 색=2:1)(RB) 및 백색광(W)에서 130μmol·m^-2·s^-1 PPFD 광도로 25일간 재배하였다. 정식 후 일주일 간격으로 25 일 동안 엽장, 엽폭, 엽병, 엽수, 광합성률을 측정하였고, 상편생장지수(leaf epinasty index, LEI)는 잎이 최대로 전개된 후에 측정하였다. 상편생장이 발생된 잎 가운데 와 가장자리를 자른 조직의 세포길이, 폭 및 세포면적은 400배율 광학현미경을 이용하여 측정하였다. 정식 후 25 일째에는 엽면적, 뿌리길이, 지상부 및 지하부의 생체중, 건물중을 조사하였다. 지상부 생체중과 건물중, 엽수, 엽 면적 모두 월드스타 품종이 수시로 품종에 비해 유의적 으로 높았다. 건물중은 월드스타 품종의 경우 혼합광 (RB)와 적색광(R) 두 처리구에서 청색광(B)와 흰색광 (W) 두 처리보다 약 35% 유의적으로 높았다. 수시로 품 종의 경우 혼합광(RB) 처리구에서 지상부 건물중이 가 장 높아 건물중이 가장 낮았던 흰색광(W) 처리구에 비 해 40% 높은 건물중 결과를 보였다. 두 품종 모두 혼합 광(RB)와 적색광(R) 두 처리구에서만 정식 21일 이후 잎 상편생장(leaf epinasty)이 나타났고 적색광(R) 처리구 에서 혼합광(RB) 처리구 보다 유의적으로 높아 잎 상편 생장은 적색광(R)과 관련이 있는 것을 알 수 있었다. 잎 가운데와 가장자리 부위 세포크기를 현미경으로 관찰한 결과 두 품종 모두 상편생장이 나타난 적색광(R) 처리구 의 잎 가장자리 세포밀도가 잎 가운데 보다 낮은 것으 로 나타나 앞서 보고된 연구결과들에서 제시한 상편생장 과 잎 가운데와 가장자리 부위의 세포크기 차이 연관성 을 뒷받침하고 있다. 또한 청색광(B)이 적색광(B)에서 발생되는 상편생장을 완화시켜주는 역할을 하는 것으로 보여 앞으로 두 광원의 적절한 혼합비율 규명이 필요한 것으로 판단된다. 또한, 엽형 변화가 심했던 수시로 품 종보다는 월드스타 품종이 LED 광원을 이용한 식물공 장 재배에 더 적합한 것으로 판단된다.

키워드

광형태형성 반응

상편생장지수

광합성율

비엽중

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Information

Publisher :The Korean Society for Bio-Environment Control
Publisher(Ko) :(사)한국생물환경조절학회
Journal Title :Protected Horticulture and Plant Factory
Journal Title(Ko) :시설원예ㆍ식물공장
Volume : 27
No :3
Pages :222-230
Received Date : 2018-05-16
Revised Date : 2018-06-30
Accepted Date : 2018-07-03
DOI :https://doi.org/10.12791/KSBEC.2018.27.3.222

Journal of Bio-Environment Control ISSN:1229-4675(Print) 2765-3641(Online) 생물환경조절학회지

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