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2026 Vol.35, Issue 3 Preview Page

Original Articles

Use of Microbial inoculants to Alleviate Soil Salinity and Improve Summer Squash Growth in Greenhouse Cultivation

염류장애 완화를 위해 미생물제제 사용을 통한 온실 애호박 생장증진 효과

Gimin Im1
,
Heewoong Goo2
,
Gyuwon Lee3
,
Wookjin Song4
,
Hyun Mun Kim5
,
Kyoung Sub Park6*
임 기민1
,
구 희웅2
,
이 규원3
,
송 욱진4
,
김 현문5
,
박 경섭6*
1Graduate Student, Department of Horticulture science, Mokpo National University, Muan 58554, Korea
2Graduate Student, Graduate School of Horticulture, Chiba University, Chiba 271-8510, Japan
3Researcher, IT Application Research Center, Korea Electronics Technology Institute, Jeonju 54853, Korea
4Researcher, Jeollanam-do Agricultural Research & Extension Services, Naju 58213, Korea
5Researcher, Global Smart Agribusiness Research Center, Muan 58554, Korea
6Professor, Department of Horticulture science, Mokpo National University, Muan 58554, Korea
1국립목포대 원예과학과 대학원생
2치바대 원예학과 대학원생
3전자기술연구원 전북지역본부 연구원
4전남농업기술원 원예연구소 농업연구사
5국립목포대 글로벌스마트농업연구소 연구원
6국립목포대 원예과학과 교수
*Corresponding Author
31 July 2026. pp. 203-213
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Abstract

Greenhouse soil cultivation is often affected by salt accumulation due to limited water exchange and intensive fertigation, which deteriorates soil structure and restricts crop performance. This study aimed to evaluate whether microbial inoculants could alleviate salinity stress by improving soil properties, thereby enhancing the growth and productivity of summer squash under greenhouse conditions. The study was conducted from May to November 2023 in Baeksan-myeon, Buan-gun, Republic of Korea. Among ten greenhouses at the site, four were selected and assigned to either an untreated control or a microbial inoculant (MI) treatment composed of Azotobacter vinelandii, Burkholderia vietnamiensis, Paenibacillus polymyxa, and Pseudomonas putida (>106 CFU mL-1 each). The MIs were applied by soil drenching at 14-day intervals from mid-May to late November 2023. Soil physical properties, soil chemical properties, plant growth traits, and fruit yield were measured. MI application improved soil physical conditions by reducing soil hardness and bulk density. Soil electrical conductivity and ammonium nitrogen concentrations were lower in the treatment group than in the control. Most other chemical properties, including phosphorus and calcium concentrations, showed no significant differences between the treatment and control groups. Early-stage vegetative growth was enhanced by MI application; however, late-stage plant height and stem diameter did not differ markedly between the two groups. Nevertheless, fruit yield was higher in the treatment group than in the control. MIs improved soil physical conditions and were associated with reduced salinity stress indicators, supporting enhanced summer squash performance and increased yield under greenhouse cultivation. These findings suggest that MIs can be used as a sustainable soil management option in intensive greenhouse systems.

Keywords
Cucurbita moschata D.
greenhouse soil cultivation
salinity stress
soil fertility
soil microbial amendment

온실 재배 토양은 제한된 수분 교환과 과도한 비료 공급으로 인해 염류 집적이 되기 쉽습니다. 염류집적은 토양 구조를 악화시키고, 작물의 생육을 저해합니다. 본 연구는 미생물 접종제가 토양 특성을 개선하여 염분 스트레스를 완화하고, 온실 애호박의 생장과 생산성을 향상시킬 수 있는지를 평가하는 것을 목표로 진행하였습니다. 실험은 2023년 5월부터 11월까지 대한민국 부안군 백산면에서 수행되었습니다. 해당 부지의 온실 10개 중 4개를 선정하여 무처리 대조군과 아조토박터, 슈도모나스 등(각각 >106 CFU mL-1)으로 구성된 복합미생물제제(MI) 처리군으로 나누어 처리하였습니다. 복합미생물제제(MI)는 2023년 5월 중순부터 11월 말까지 14일 간격으로 토양 관주 방식으로 처리했습니다. 토양 물리적 특성, 토양 화학적 특성, 식물 생장 특성 및 과실 수확량을 측정했습니다. MI 처리는 토양 경도와 용적 밀도를 감소시켜 토양 물리적 조건을 개선했습니다. 토양 전기 전도도와 질소산암모늄 농도는 처리군이 대조군보다 낮았습니다. 인과 칼슘 농도를 포함한 대부분의 다른 화학적 특성은 처리군과 대조군 사이에 유의미한 차이를 보이지 않았습니다. MI 처리는 초기 영양 생장을 촉진했지만, 후기 식물 높이와 줄기 직경은 두 군 간에 뚜렷한 차이가 없었습니다. 그럼에도 불구하고 과일 수확량은 처리군이 대조군보다 높았습니다. MI는 토양 물리적 조건을 개선하고 염분 스트레스 지표를 감소시켜 온실 재배 환경에서 여름 호박의 생육 및 수확량 증가를 도왔습니다. 이러한 결과는 MI가 염류가 집적된 온실에서 지속 가능한 토양 관리 방안으로 활용될 수 있음을 시사합니다.

키워드
미생물 토양개량제
애호박
염류 스트레스
토양비옥도
토양재배
References
1

Adriansyah D, Kusmiyati F (2020) Growth and production of determinate and indeterminate soybean (Glycine max L.) influenced by salinity stress. E3S Web of Conferences 142:03004. https://doi.org/10.1051/e3sconf/202014203004

10.1051/e3sconf/202014203004
2

Alori ET, Dare MO, Babalola OO (2017) Microbial inoculants for soil quality and plant health. In E. Lichtfouse, ed, Sustainable Agriculture Reviews, Vol 22. Springer, Cham, pp 281-307. https://doi.org/10.1007/978-3-319-48006-0_9

10.1007/978-3-319-48006-0_9
3

Al-Shammary AAG, Kouzani AZ, Kaynak A, Khoo SY, Norton M, Gates W (2018) Soil bulk density estimation methods: A review. Pedosphere 28:581-596. https://doi.org/10.1016/S1002-0160(18)60034-7

10.1016/S1002-0160(18)60034-7
4

Blanco FF, Folegatti MV (2002) Salt accumulation and distribution in a greenhouse soil as affected by salinity of irrigation water and leaching management. Rev Bras Eng Agric Ambient 6:414-419. https://doi.org/10.1590/S1415-43662002000300006

10.1590/S1415-43662002000300006
5

Calvo P, Nelson L, Kloepper JW (2014) Agricultural uses of plant biostimulants. Plant Soil 383:3-41. https://doi.org/10.1007/s11104-014-2131-8

10.1007/s11104-014-2131-8
6

Calvo P, Watts DB, Kloepper JW, Torbert HA (2017) Effect of microbial‐based inoculants on nutrient concentrations and early root morphology of corn (Zea mays). J Plant Nutr Soil Sci 180:56-70. https://doi.org/10.1002/jpln.201500616

10.1002/jpln.201500616
7

Cameron K, Di HJ, Moir J (2013) Nitrogen losses from the soil/plant system: a review. Ann Appl Biol 162:145-173. https://doi.org/10.1111/aab.12014

10.1111/aab.12014
8

Carvajal M., Martínez V, Alcaraz CF (1999) Physiological function of water channels as affected by salinity in roots of paprika pepper. Physiol Plant 105:95-101. https://doi.org/10.1034/j.1399-3054.1999.105115.x

10.1034/j.1399-3054.1999.105115.x
9

Chen X., Yaa O-K, Wu J (2020) Effects of different organic materials application on soil physicochemical properties in a primary saline-alkali soil. Eurasian Soil Sci 53:798-808. https://doi.org/10.1134/S1064229320060034

10.1134/S1064229320060034
10

Egamberdiyeva D (2007) The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Appl Soil Ecol 36:184-189. https://doi.org/10.1016/j.apsoil.2007.02.005

10.1016/j.apsoil.2007.02.005
11

Gee GW, Bauder JW (1986) Particle‐size analysis. In Arnold Klute, Methods of soil analysis: Part 1 Physical and mineralogical methods 5, the American Society of Agronomy, Madison, pp 383-411. https://doi.org/10.2136/sssabookser5.1.2ed.c15

10.2136/sssabookser5.1.2ed.c15
12

Goo HW, Kim EJ, Na HY, Park KS (2022) Effects of Heating Initiative Temperature and CO2 Fertilizing Concentration on the Growth and Yield of Summer Squash in a Greenhouse. J Bio-Environ Control 31:468-475. https://doi.org/10.12791/KSBEC.2022.31.4.468

10.12791/KSBEC.2022.31.4.468
13

Gyaneshwar P, Naresh Kumar G, Parekh L, Poole P (2002) Role of soil microorganisms in improving P nutrition of plants. Plant Soil 245:83-93. https://doi.org/10.1023/A:1020663916259

10.1023/A:1020663916259
14

Ha S-K, Sonn Y-K, Jung K-H, Lee Y-J, Cho M-J, Yun H-J, Sung J-K (2015) Estimation of growth stage-based nitrogen supply levels for greenhouse semi-forcing zucchini cultivation. Korean J Agric Sci 42:319-324. https://doi.org/10.7744/cnujas.2015.42.4.319

10.7744/cnujas.2015.42.4.319
15

Hemkemeyer M, Schwalb SA, Heinze S, Joergensen RG, Wichern F (2021) Functions of elements in soil microorganisms. Microbiol Res 252:126832. https://doi.org/10.1016/j.micres.2021.126832

10.1016/j.micres.2021.126832
16

Horn R, Taubner H, Wuttke M, Baumgartl T. (1994) Soil physical properties related to soil structure. Soil Tillage Res 30:187-216. https://doi.org/10.1016/0167-1987(94)90005-1

10.1016/0167-1987(94)90005-1
17

Huang L-F, Song L-X, Xia X-J, Mao W-H, Shi K, Zhou Y-H, Yu J-Q (2013) Plant-soil feedbacks and soil sickness: from mechanisms to application in agriculture. J Chem Ecol 39:232-242. https://doi.org/10.1007/s10886-013-0244-9

10.1007/s10886-013-0244-9
18

Katerji N, Van Hoorn J, Hamdy A, Mastrorilli M (1998) Response of tomatoes, a crop of indeterminate growth, to soil salinity. Agric Water Manag 38:59-68. https://doi.org/10.1016/S0378-3774(98)00051-1

10.1016/S0378-3774(98)00051-1
19

KOSIS (2020) Agriculture Crop Production Survey: Fruit Vegetable. https://www.k-stat.go.kr/metasvc/msba100/statsdcdta?statsConfmNo=114018&kosisYn=Y Accessed 10 June 2026

20

Lee S-B, Sung J-K, Lee Y-J, Lim J-E, Song Y-S, Lee D-B, Hong S-Y (2017) Analysis of soil total nitrogen and inorganic nitrogen content for evaluating nitrogen dynamics. Korean J Soil Sci Fert 50:100-105. https://doi.org/10.7745/KJSSF.2017.50.2.100

10.7745/KJSSF.2017.50.2.100
21

Liu X, Zhang Y, Ren X, Chen B, Shen C. Wang F (2019) Long-term greenhouse vegetable cultivation alters the community structures of soil ammonia oxidizers. J Soil Sediment 19:883-902. https://doi.org/10.1007/s11368-018-2089-x

10.1007/s11368-018-2089-x
22

Meena VS, Maurya B, Verma JP (2014) Does a rhizospheric microorganism enhance K+ availability in agricultural soils? Microbiol Res 169:337-347. https://doi.org/10.1016/j.micres.2013.09.003

10.1016/j.micres.2013.09.003
23

Ondrasek G, Rengel Z, Veres S (2011) Soil salinisation and salt stress in crop production. In A. Shanker and B. Venkateswarlu, eds, Abiotic Stress in Plants: Mechanisms and Adaptations. InTech, Rijeka, pp 171-190. https://doi.org/10.5772/22248

10.5772/22248
24

Park KS, Huh YC, Lee HE, Park DK, Kwon JK (2010) Effect of Plastic Bagging Cultivation of Summer Squash (Cucurbita moschata) on Improvement of Quality and Extension of Storage Period. J Bio-Environ Control 19:372-376.

25

Rawat A, Rao D, Sahu R (2013) Effect of soybean inoculation with Bradyrhizobium and wheat inoculation with Azotobacter on their productivity and N turnover in a Vertisol. Arch Agron Soil Sci 59:1559-1571. https://doi.org/10.1080/03650340.2012.740555

10.1080/03650340.2012.740555
26

Rhoades JD (1996) Salinity: Electrical conductivity and total dissolved solids. In DL Sparks, AL Page, PA Helmke, RH Loeppert, PN Soltanpour, MA Tabatabai, CT Johnston, and ME Sumner, eds, Methods of Soil Analysis: Part 3 Chemical Methods. SSSA Book Series 5. Soil Science Society of America, Madison, WI, pp 417-435. https://doi.org/10.2136/sssabookser5.3.c14

10.2136/sssabookser5.3.c14
27

Shahwar D, Mushtaq Z, Mushtaq H, Alqarawi AA, Park Y, Alshahrani TS, Faizan S (2023) Role of microbial inoculants as bio fertilizers for improving crop productivity: A review. Heliyon 9:e16134. https://doi.org/10.1016/j.heliyon.2023.e16134

10.1016/j.heliyon.2023.e1613437255980PMC10225898
28

Shirazi MA, Boersma L (1984) A unifying quantitative analysis of soil texture. Soil Sci Soc Am J 48:142-147. https://doi.org/10.2136/sssaj1984.03615995004800010026x

10.2136/sssaj1984.03615995004800010026x
29

Simranjit K, Kanchan A, Prasanna R, Ranjan K, Ramakrishnan B, Singh AK, Shivay YS (2019) Microbial inoculants as plant growth stimulating and soil nutrient availability enhancing options for cucumber under protected cultivation. World J Microbiol Biotechnol 35:51. https://doi.org/10.1007/s11274-019-2623-z

10.1007/s11274-019-2623-z
30

Song Y, Xu M, Li X, Bian Y, Wang F, Yang X, Gu C, Jiang X (2018) Long-term plastic greenhouse cultivation changes soil microbial community structures: a case study. J Agric Food Chem 66:8941-8948. https://doi.org/10.1021/acs.jafc.8b01829

10.1021/acs.jafc.8b01829
31

Sparks DL, Page AL, Helmke PA, Loeppert RH (2020) Methods of Soil Analysis: Part 3 Chemical Methods. John Wiley & Sons, Hoboken, NJ.

32

Tighe M, Lockwood P, Wilson S, Lisle L (2004) Comparison of digestion methods for ICP-OES analysis of a wide range of analytes in heavy metal contaminated soil samples with specific reference to arsenic and antimony. Commun Soil Sci Plant Anal 35:1369-1385. https://doi.org/10.1081/CSS-120037552

10.1081/CSS-120037552
33

Trabelsi D, Mhamdi R (2013) Microbial inoculants and their impact on soil microbial communities: a review. BioMed Res Int 2013:863240. https://doi.org/10.1155/2013/863240

10.1155/2013/86324023957006PMC3728534
34

Tsavkelova E, Klimova SY, Cherdyntseva T, Netrusov A (2006) Hormones and hormone-like substances of microorganisms: a review. Appl Biochem Microbiol 42:229-235. https://doi.org/10.1134/S000368380603001X

10.1134/S000368380603001X
35

Vı́llora G, Moreno DA, Pulgar G, Romero L (2000) Yield improvement in zucchini under salt stress: determining micro-nutrient balance. Sci Hortic 86:175-183. https://doi.org/10.1016/S0304-4238(00)00149-7

10.1016/S0304-4238(00)00149-7
36

Wang C, Luo Y, Huo Z, Liu Z, Liu G, Wang S, Lin Y, Wu P (2022) Salt accumulation during cropping season in an arid irrigation area with shallow water table depth: a 10-year regional monitoring. Water 14:1664. https://doi.org/10.3390/w14101664

10.3390/w14101664
37

Wang E, Cruse RM, Zhao Y, Chen X (2015) Quantifying soil physical condition based on soil solid, liquid and gaseous phases. Soil Tillage Res 146:4-9. https://doi.org/10.1016/j.still.2014.09.018

10.1016/j.still.2014.09.018
38

Welbaum GE, Sturz AV, Dong Z, Nowak J (2004) Managing soil microorganisms to improve productivity of agro-ecosystems. Crit Rev Plant Sci 23:175-193. https://doi.org/10.1080/07352680490433295

10.1080/07352680490433295
39

Xu H-L, Wang R, Mridha MAU (2000) Effects of organic fertilizers and a microbial inoculant on leaf photosynthesis and fruit yield and quality of tomato plants. J Crop Prod 3:173-182. https://doi.org/10.1300/J144v03n01_15

10.1300/J144v03n01_15
Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Journal of Bio-Environment Control
  • Journal Title(Ko) :생물환경조절학회지
  • Volume : 35
  • No :3
  • Pages :203-213
  • Received Date : 2026-04-11
  • Revised Date : 2026-06-10
  • Accepted Date : 2026-06-15
  • DOI :https://doi.org/10.12791/KSBEC.2026.35.3.203
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