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2017 Vol.26, Issue 4 Preview Page
Rapid Analysis of Nitrate Concentration in Different Growth Stages and Plant Parts of Paprika Leaf Using On-site Rapid Detection Kit

신속분석기기를 이용한 파프리카 생육단계 및 부위별 엽내 질산태질소 농도 신속분석

Min Ji Lee1
,
Han Cheol Rhee2
,
Gyeong Lee Choi2
,
Sang Seok Oh3
,
Jae Taek Lee4
,
Jun Gu Lee15*
이 민지1
,
이 한철2
,
최 경이2
,
오 상석3
,
이 재택4
,
이 준구15*
Department of Horticulture, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 54896, Korea
Protected Horticulture Research Station, National Institute of Horticulture & Herbal Science, Rural Development Administration, Haman 52054, Korea
Gyeongnam Agricultural Research & Extension Services, Jinju 52733, Korea
Fruit Vegetable Research Institute, Jeonbuk ARES., Gunsan 54062, Korea
Institute of Agricultural Science & Technology, Chonbuk National University, Jeonju 54896, Korea
전북대학교 원예학과
국립원예특작과학원 시설원예연구소
경상남도농업기술원 미래농업교육과
전라북도농업기술원 과채류연구소
전북대학교 농업과학기술연구소
*Corresponding author: jungu@jbnu.ac.kr
30 November 2017. pp. 333-339
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Abstract

This research was aimed to establish rapid analysis technique for the determination of nitrate (NO3-) concentration in the leaves of paprika, which has key role for the stable vegetative and reproductive growth. Leaf petiole and blade sap of two paprika cultivars (‘Raon red’ and ‘Raon yellow’) were used for the determination of NO3- concentration, separately using rapid detection kit (RQ-flex) and spectroscopy quantification methods. In addition, two paprika cultivars namely, ‘Nicole’ and ‘TP2001’ were used to determine the status of NO3- concentration in leaf of each fruiting group. NO3- concentration in leaf blade sap and the content in leaf showed significant correlation (R2 = 0.8628), analysed by RQ-flex and spectroscopy methods, respectively. Furthermore, leaf petiole sap and the content in leaf also showed significant correlation (R2=0.6734) but the relationship was poor compared to leaf blade sap and the leaf content. NO3- concentration in petiole sap decreased in all the cultivars from early to late fruiting group. The higher concentration in the lower leaves and the continuous decrease towards the upper leaves in the both years were found through the analysis of NO3- concentration in different leaf position. In addition, daily short-term fluctuation of NO3- in petiole sap could be rapidly monitored. These results showed that long-term or short-term monitoring by test strip-based rapid analysis technique might be useful tool for the diagnosis of nutritional status for the stable of nutritional management in paprika.

Keywords
nutritional diagnosis
NO3-
petiole sap
test strip

본 연구는 겨울철 파프리카의 안정된 영양생장, 생식 생장을 파악하는 방법으로 간이영양진단기기를 이용해 NO3- 농도를 신속하게 측정하고 영양상태 파악을 위해 실시되었다. ‘Raon red’, ‘Raon yellow’ 두 품종을 각각 비대기 단계로 나누어 측정한 결과 엽신의 test strip 분 석은 분광광도계를 통한 관행의 방법과 상관성이 인정되 었고(R2=0.8628), 엽병의 test strip분석은 엽신 즙액 보 다는 낮은 상관성을 보이지만 간접적으로 NO3- 농도 분 석에 유용하였다(R2=0.6734). 착과 그룹이 높아짐에 따 라 해당 부위 엽 내 질산태질소 농도가 낮아지는 경향 을 보이고 있으며, 동일 시점의 마디별 엽병 즙액 내 질 산태질소 농도 역시 정단부로 갈수록 낮아지는 경향이 확인되었다. 엽병 즙액 내 NO3- 농도 역시 간이진단기기 를 활용하여 신속한 모니터링이 가능하였다. 따라서 간 이영양진단기기를 사용해 기존 분석방법보다 효과적으로 농가 현장에서 파프리카의 질소 영양상태를 파악할 수 있었으며, 이를 통해 추후 합리적인 양액공급 및 표준영 양범위의 설정이 가능할 것으로 판단된다.

키워드
영양진단
NO3-
엽병즙액
Test strip
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Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Protected Horticulture and Plant Factory
  • Journal Title(Ko) :시설원예ㆍ식물공장
  • Volume : 26
  • No :4
  • Pages :333-339
  • Received Date : 2017-08-11
  • Revised Date : 2017-09-05
  • Accepted Date : 2017-10-11
  • DOI :https://doi.org/10.12791/KSBEC.2017.26.4.333
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