Application of Chlorophyll Fluorescence Parameters to Diagnose Salinity Tolerance in the Seedling of Tomato Genetic Resources

Yu Kyeong Shin, Jung Su Jo, Myeong-Cheoul Cho, Eun-Young Yang, Yul Kyun Ahn, In deok Hwang, and Jun Gu Lee

doi:10.12791/KSBEC.2021.30.2.165

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2021 Vol.30, Issue 2 Preview Page

Original Articles

Application of Chlorophyll Fluorescence Parameters to Diagnose Salinity Tolerance in the Seedling of Tomato Genetic Resources 엽록소형광 매개변수를 이용한 토마토 유전자원의 유묘 단계 염류 스트레스 수준 평가

April 2021. pp. 165-173

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Abstract

본 연구에서는 토마토 유전자원 49점을 대상으로 엽록소형광 측정 프로토콜인 Quenching act 2를 이용하여 염류 스트레스 수준을 구분할 수 있는 엽록소형광 매개변수를 선발하기 위해 수행되었다. 염류 스트레스 평가는 3엽기 유묘 단계의 토마토 유전자원 49점을 대상으로, 1일 1회 NaCl 400mM로 저면관수를 처리하였다. 염류 스트레스 처리 4일차에 토마토 유묘의 지상부 생체중, 엽록소 및 프롤린 함량 분석을 실시하였다. 토마토 유전자원 49점의 지상부 생체중 및 엽록소 함량은 대부분 감소하였으며, 프롤린 함량 증가율은 유전자원 별로 유사하였다. 대표적인 12개의 엽록소형광 매개변수는 염류 스트레스에 노출되는 기간이 길어질수록 증감하는 경향을 보였으며, 염류 스트레스에 노출될수록 Y(NO)는 증가하였다. 본 연구결과에서 유전자원 49점의 광계Ⅱ의 비조절 에너지 소산의 양자 수율[Y(NO)]은 염류 스트레스 하에 차이를 보였으며, 염류 스트레스에 저항성을 지닌 염류 저항성 유전자원과 염류 스트레스에 감수성 유전자원 간의 차이를 확인할 수 있는 엽록소형광 매개변수로 판단되며, 토마토 유전자원에 대해 염류 스트레스 수준을 평가할 수 있는 보완적인 도구로 활용 가능하다고 판단된다.

키워드

비파괴진단

유전자원 평가

염류 스트레스

엽록소형광

프롤린

To evaluate the possibility of a non-destructive diagnosis of salinity stress in the tomato genetic resources using chlorophyll fluorescence (CF) imaging technique, 49 tomato genetic resources at 3-leaf stages were used in this study. The seedlings were irrigated once a day with tap water and 400 mM NaCl for control and salinity stress treatment, respectively and the CF parameters were assessed during four days of the experimental period. The shoots were collected and used for the measurement of growth parameters, chlorophyll and proline contents at the end of the treatment. The chlorophyll content (a and b) and fresh weight showed differential changes (%) among the susceptible, moderately resistant, and resistant genetic resources, while changes in the proline content were similar in all the genetic resources. All the CF parameters showed a positive or negative response to the salinity stress in which the quantum yield of non-regulated energy dissipation in PSII [Y(NO)] continuously increased regardless of the treatment time in the resistant, moderately resistant and susceptible genetic resources. Y(NO) was used for the screening of the 49 genetic resources and the result showed that the clear differences in Y(NO) among the susceptible, moderately resistant, and resistant genetic resources. It is concluded that the Y(NO) can be used as a CF parameter index for the screening of salinity stress tolerance in tomato genetic resources.

Keywords

chlorophyll content

proline

salinity stress

tomato genetic resource

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Information

Publisher :The Korean Society for Bio-Environment Control
Publisher(Ko) :(사)한국생물환경조절학회
Journal Title :Journal of Bio-Environment Control
Journal Title(Ko) :생물환경조절학회지
Volume : 30
No :2
Pages :165-173
Received Date :2021. 04. 07
Revised Date :2021. 04. 28
Accepted Date : 2021. 04. 28
DOI :https://doi.org/10.12791/KSBEC.2021.30.2.165

Journal of Bio-Environment Control ISSN:1229-4675(Print) 2765-3641(Online) 생물환경조절학회지

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