Sterilizing Effect of Plant Pathogenic Fungi using Ozone Microbubble

Chang Shoo Kim; Sang Yeol Yu; Gong In Lee; Seung Han Kim; Jong Won Lee; Jae Kwan Song

doi:10.12791/KSBEC.2014.23.3.250

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2014 Vol.23, Issue 3 Preview Page Next Page

Sterilizing Effect of Plant Pathogenic Fungi using Ozone Microbubble 오존마이크로버블을 이용한 식물병원균 살균효과

30 July 2014. pp. 250-255

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Abstract

Sterilization is an important factor in reprocessing of drained nutrient solution (RDN). To evaluate the suitability of ozone microbubble for RDN in protected horticulture and plant factory, strong fungicidal activity of ozone and microbubble were applied. This experiment was taken advantage of ozonated water (OW) and ozone gas (OG). The Fusarium oxysporum (FO), Phytophthora capsici (PC) and Colletotrichum gloeosporioides (CG) were treated with OW 0.5, 1.0 and 2.0 ppm and OG 3.0 g·h⁻¹ for 0, 30, 60, 120 and 180 sec. Results of this experiment can be summed up as follows : In the OW, FO was sterilized by 0.5 ppm in 60 sec and PC was sterilized by 2.0 ppm in 30 sec. In the OG, FO and PC both of them were sterilized in 180 sec. However, CG was not sterilized using OW and OG. Overall, ozone microbubble showed possibility of sterilization in RDN. However, CG is required to more ozone concentration and processing time.

Keywords

reprocessing

nutrient solution

ozonated water

ozone gas

fungicidal activity

본 연구는 살균 소독제로 오존을 이용하고. 살균효과 를 극대화 할 수 있는 방식으로 마이크로버블 장치에 오존을 공급하여 배양액 재처리 기술을 위한 오존 마이 크로버블의 살균효과를 구명하고자 실시하였으며 결과를 요약하면 다음과 같다.

마이크로버블 장치의 성능에서 압력 3.5kgf·cm⁻²에서 평균입경은 27.42μm로 측정되었고 마이크로버블의 발생 량은 당 평균 12만개로 나타났으며, 적정 오존 발생량은 3g·h⁻¹일 때 오존농도는 2ppm, 배출 오존농도는 0.06ppm에 도달하는 것으로 나타났다.

병원균 살균효과는 오존수의 경우 FO, PC 와 CG 모 두 오존농도 0.5ppm, 처리시간 30초만으로도 현저한 감 소를 보였다. FO는 오존농도 0.5ppm, 처리시간 60초 이 내에서 멸균되었고, PC는 오존농도 2.0ppm, 처리시간 30초 이내에서 멸균되었으나, CG의 경우 2.0ppm 이상 의 오존수를 처리하여야 할 것으로 판단되었다. 오존가 스의 경우 처리시간 120초 이내에서 현저한 감소를 보 였으며, FO와 PC는 처리시간 180초 이내에서 멸균되었 고, CG의 경우 180초 이상 오존가스를 처리하여야 할 것으로 판단되었다.

키워드

재처리

배양액

오존수

오존가스

살균력

References

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Information

Publisher :The Korean Society for Bio-Environment Control
Publisher(Ko) :(사)한국생물환경조절학회
Journal Title :Protected Horticulture and Plant Factory
Journal Title(Ko) :시설원예ㆍ식물공장
Volume : 23
No :3
Pages :250-255
Received Date : 2014-08-26
Revised Date : 2014-09-04
Accepted Date : 2014-09-15
DOI :https://doi.org/10.12791/KSBEC.2014.23.3.250

Journal of Bio-Environment Control ISSN:1229-4675(Print) 2765-3641(Online) 생물환경조절학회지

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