Diagnosis of Nitrogen Content in the Leaves of Apple Tree Using Spectral Imagery

Si Hyeong Jang; Jung Gun Cho; Jeom Hwa Han; Jae Hoon Jeong; Seul Ki Lee; Dong Yong Lee; Kwang Sik Lee

doi:10.12791/KSBEC.2022.31.4.384

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2022 Vol.31, Issue 4 Preview Page Next Page

Original Articles

Diagnosis of Nitrogen Content in the Leaves of Apple Tree Using Spectral Imagery 분광 영상을 이용한 사과나무 잎의 질소 영양 상태 진단

31 October 2022. pp. 384-392

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Abstract

The objective of this study was to estimated nitrogen content and chlorophyll using RGB, Hyperspectral sensors to diagnose of nitrogen nutrition in apple tree leaves. Spectral data were acquired through image processing after shooting with high resolution RGB and hyperspectral sensor for two-year-old ‘Hongro/M.9’ apple. Growth data measured chlorophyll and leaf nitrogen content (LNC) immediately after shooting. The growth model was developed by using regression analysis (simple, multi, partial least squared) with growth data (chlorophyll, LNC) and spectral data (SPAD meter, color vegetation index, wavelength). As a result, chlorophyll and LNC showed a statistically significant difference according to nitrogen fertilizer level regardless of date. Leaf color became pale as the nutrients in the leaf were transferred to the fruit as over time. RGB sensor showed a statistically significant difference at the red wavelength regardless of the date. Also hyperspectral sensor showed a spectral difference depend on nitrogen fertilizer level for non-visible wavelength than visible wavelength at June 10th and July 14th. The estimation model performance of chlorophyll, LNC showed Partial least squared regression using hyperspectral data better than Simple and multiple linear regression using RGB data (Chlorophyll R²: 81%, LNC: 81%). The reason is that hyperspectral sensor has a narrow Full Half at Width Maximum (FWHM) and broad wavelength range (400－1,000 nm), so it is thought that the spectral analysis of crop was possible due to stress cause by nitrogen deficiency. In future study, it is thought that it will contribute to development of high quality and stable fruit production technology by diagnosis model of physiology and pest for all growth stage of tree using hyperspectral imagery.

Keywords

hyperspectral imagery

leaf nutrition

nitrogen fertilizer

linear regression

growth model

본 연구는 RGB, 초분광 센서를 이용하여 시기별 사과 잎의 엽록소와 질소 함량을 예측하여 사과 나무 잎의 질소 영양을 진단하기 위해 수행되었다. 분광 데이터는 사과나무 ‘홍로/M.9’ 2년생을 대상으로 고해상도 RGB와 초분광 센서로 촬영 후 영상처리를 통해 취득하였다. 식물체 데이터는 촬영이 끝난 직후 엽록소와 잎 질소 함량을 측정하였다. 엽록소 측정기의 SPAD meter, RGB 센서의 개별 파장, 컬러 식생지수 및 초분광 센서의 214개의 파장과 식물체 데이터를 이용하여 회귀분석을 실시하였다. 엽록소와 잎 질소 함량 데이터는 시기와 상관없이 질소 시비량에 따라 통계적으로 유의한 차이가 나타났다. 잎은 시기가 지나면서 잎에 있던 영양분이 과실로 전이되어 색이 옅어졌으며 RGB센서의 경우 Red파장에서 시기와 상관없이 통계적으로 유의한 차이가 나타났다. 초분광 센서의 경우 두 시기 모두 질소 시비 수준에 따라 가시광 영역보다 비가시광 영역에서 차이가 크게 나타났다. 반사값를 이용하여 식물체 특성의 예측 모델 결과 엽록소, 잎 질소함량 모두 초분광 데이터를 이용한 부분최소제곱 회귀분석을 이용하였을 때 성능이 가장 높게 나타났다(chlorophyll: 81% / 63%, leaf nitrogen content: 81% / 67%). 이러한 원인은 RGB 센서에 비해 초분광 센서는 좁은 FWHM과 400－1,000nm의 넓은 파장 범위를 가지고 있어 질소 결핍에 의한 스트레스로 인해 작물의 분광학적 해석이 가능했을 것으로 판단된다. 추후 분광학적 특성을 이용하여 전 생육 시기의 수체 생리, 생태 모델 개발 및 검증 그리고 병해충 진단 등 연구를 통해 고품질, 안정적인 과실 생산 기술 개발에 기여될 것으로 사료된다.

키워드

사과 나무 잎

선형 회귀

생육 모델

질소 함량

초분광 영상

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Information

Publisher :The Korean Society for Bio-Environment Control
Publisher(Ko) :(사)한국생물환경조절학회
Journal Title :Journal of Bio-Environment Control
Journal Title(Ko) :생물환경조절학회지
Volume : 31
No :4
Pages :384-392
Received Date : 2022-09-23
Revised Date : 2022-10-18
Accepted Date : 2022-10-18
DOI :https://doi.org/10.12791/KSBEC.2022.31.4.384

Journal of Bio-Environment Control ISSN:1229-4675(Print) 2765-3641(Online) 생물환경조절학회지

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