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Expression of Genes Affecting Skin Coloration and Sugar Accumulation in ‘Hongro’ Apple Fruits at Ripening Stages in High Temperatures

고온에 의한 변색단계별 ‘홍로’ 사과의 착색 및 당 축적 관련 유전자 발현 분석

Seon Ae Kim1
,
Soon Young Ahn1
,
Hae Keun Yun1*
김 선애1
,
안 순영1
,
윤 해근1*
1Department of Horticulture and Life Science, Yeungnam University, Gyeongsan 38541, Korea
1영남대학교 원예생명과학과
*Corresponding author: haekeun@ynu.ac.kr
31 January 2016. pp. 9-15
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Abstract

High temperature is one of the important environmental factors limiting cultivation of apple (Malus domestica Borkh). The expression of genes related with anthocyanin synthesis and sugar accumulation in response to high temperature was studied in the ‘Hongro’ apple fruits at different developmental stages in different temperature conditions through real-time PCR. Expression of â-amylase (BMY) and polygalacturonase (PG) genes related with sugar synthesis was higher in late ripening stages than in initial ripening stages. Expression of four genes such as phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and malate dehydrogenase (MDH), which were related with fruit skin coloration, increased gradually in apple fruits of the middle and late ripening stages. Interestingly, the expressions of all genes were highly inhibited expressed at 30-35°C compared to 25°C in all ripening stages. In the further work, investigation of expression levels of various genes could be conducted in the level of transcriptomics in fruits at the middle ripening stages to get meaningful information of ripening metabolism in apple in high temperatures.

Keywords
anthocyanin
high temperature
real-time PCR
ripening metabolism

고온은 사과(Malus domestica Borkh) 과실의 품질에 영향을 끼치는 가장 중요한 환경 요인 중 하나이다. 착 색기의 ‘홍로’ 사과 과실을 3단계로 구분하여 Real-time PCR을 통해 온도조건에 따른 안토시아닌 합성과 당 축 적 관련 유전자의 발현 차이를 조사하였다. 당 합성관련 유전자인 β-amylase(BMY)와 polygalacturonase(PG)의 발 현은 변색 시작단계보다 마지막 단계에서 월등히 높았다. 과피의 착색과 관련있는 phenylalanine ammonia-lyase(PAL), chalcone synthase(CHS), flavanone 3-hydroxylase(F3H)와 malate dehydrogenase(MDH)유전자는 변색 초기작단계에 서는 고온 처리 24시간후에 발현이 증가하는 경향을 보 였으며 변색 중간단계에서는 점차 증가하는 경향을 보였 다. 변색단계별로 보았을 때 25°C처리구가 다른 온도처리 구보다 발현 정도가 더 높았으며, 변색 시작단계보다 마 지막 단계에서의 발현이 강하게 유도되었다. 본 연구의 결과로 착색 초기단계의 과실이 고온스트레스가 가장 영 향을 끼치므로 착색초기단계의 과실을 이용하여 전사체 를 분석하면 분자생물학적 수준에서 사과의 성숙대사에 서 유용한 정보를 얻을 수 있을 것으로 사료된다.

키워드
고온
성숙대사
안토시아닌
real-time PCR
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Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Protected Horticulture and Plant Factory
  • Journal Title(Ko) :시설원예ㆍ식물공장
  • Volume : 25
  • No :1
  • Pages :9-15
  • Received Date : 2016-02-08
  • Revised Date : 2016-03-23
  • Accepted Date : 2016-03-23
  • DOI :https://doi.org/10.12791/KSBEC.2016.25.1.9
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