All Issue

2024 Vol.33, Issue 1 Preview Page

Original Articles

The Effect of Gibberellin Dipping Concentration and Treatment Time on the Growth of Cutting Propagules in Strawberry

딸기 삽목 육묘 시 묘 생육에 미치는 지베렐린 침지농도 및 시간의 영향

Eun Ji Kim1
,
Chi Seon Kim2
,
Hyun Soo Jung1
,
Jun Gu Lee3*
김 은지1
,
김 치선2
,
정 현수1
,
이 준구3*
1Researcher, Jeollabuk-do Agricultural Research and Extension Services, Iksan 54591, Korea
2Senior Researcher, Jeollabuk-do Agricultural Research and Extension Services, Iksan 54591, Korea
3Professor, Department of Horticulture, College of Agriculture & Life Sciences, Jeonbuk National University, Jeonju 54896, Korea
1전라북도농업기술원 지방농업연구사
2전라북도농업기술원 지방농업연구관
3전북대학교 농업생명과학대학 원예학과 교수
*Corresponding Author
31 January 2024. pp. 12-21
PDF XML Citation
Abstract

The aim of this research was to investigate the effect of gibberellin on improving seedling growth characteristics and enhancing strawberry quality in cutting propagation. Cuttings of the cultivar ‘Seolhyang’ were treated with GA3 for 30 and 60 minutes at concentrations of 50, 100, and 150 mg·L-1, with distilled water used for dipping as the control. Evaluation of seedling growth showed a positive correlation between the duration of gibberellin dipping and growth characteristics such as leaf number and SPAD value. Plant height, petiole length, leaf length and width, and leaf area varied significantly based on the interaction between dipping time and concentration. Crown diameter exhibited differences depending on the dipping time, with cuttings producing superior seedlings having a diameter of 8.0 mm or more for all treatments except the 30-minute, 100 mg·L-1 treatment. The T/R ratio was significantly lower in the 30-minute, 50 mg·L-1 treatment, indicating the highest plant vigor. Quantum yield was lower at a concentration of 150 mg·L-1, showing a decreasing trend with increasing gibberellin concentration. Nonphotochemical quenching was significantly smaller in the 30-minute, 150 mg·L-1 treatment, indicating an effective reduction of stress in the cuttings. Antioxidant content was highest in the 30-minute, 50 mg·L-1 treatment and the 60-minute, 150 mg·L-1 treatment. Moreover, the results of post-transplanting growth assessment showed no negative effect of gibberellin on flowering induction. Therefore, it was confirmed that gibberellin treatment during the cutting propagation of ‘Seolhyang’ strawberries had a positive effect on the production of high-quality seedlings. Dipping the cuttings in 50 mg·L-1 gibberellin for 30 minutes is considered to be the most suitable method for improving growth and quality compared to the control.

Keywords
cutting propagation
forcing culture
GA3
plant growth regulator
seedling quality

본 연구는 딸기의 삽목 육묘 시 묘소질을 향상시키고 품질을 증진시키기 위한 지베렐린 효과를 검증하기 위해 수행되었다. 품종은 ‘설향’을 대상으로 하였고, 삽목 시 삽수의 기부를 30, 60분간 GA3 50, 100, 150mg·L-1에 침지 처리하고 증류수 침지를 대조구로 두었다. 정식 시 묘소질 조사 결과 엽수와 SPAD 값은 지베렐린 침지시간이 길수록 증가하였고, 초장, 엽병장, 엽장, 엽폭, 엽면적은 침지시간과 농도 간의 상호작용에 따라 유의하게 값이 변하였다. 관부직경은 같은 농도라 할지라도 침지시간에 따른 차이가 나타났으며 30분, 100mg·L-1 처리를 제외하고 모두 직경이 8.0mm 이상으로 우량한 묘가 생성되었으며, T/R률은 30분, 50mg·L-1 처리에서 유의하게 작아 식물체 활력이 가장 우수했다. 양자수율은 농도가 150mg·L-1일 때 저조하였고, 지베렐린 처리 농도가 높을수록 양자수율이 감소하는 경향을 나타냈다. NPQ는 대조구 대비 30분, 150mg·L-1 처리에서 유의하게 작아 묘 스트레스 감소에 효과가 있었다. 항산화물 함량은 30분, 50mg·L-1 처리와 60분, 150mg·L-1 처리에서 높게 나타났고, 정식 후 생육조사 결과 화아분화 정도에 미치는 지베렐린의 부정적 영향은 없었다. 따라서, ‘설향’ 딸기의 삽목 육묘 시 지베렐린 처리는 정식묘 양성에 효과가 있는 것을 확인할 수 있었으며, 이때 대조구 대비 생육 증진 및 품질 향상을 위해서는 삽수 기부를 50mg·L-1의 지베렐린에 30분 침지처리하는 것이 보다 적정한 것으로 판단되었다.

키워드
묘소질
삽목
식물생장조절제
촉성재배
GA3
References
1
Ahn C.K., J.C. Park, and I.C. Yu 1988, Effect of pot nursing in forcing culture on flower bud differentiation, growth and yield of strawberry. J Korean Hortic Soc Sci Technol 6:76-77.
2
Blois M.S. 1958, Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200. 10.1038/1811199a0
3
Chaerle L., I. Leininen, H.G. Jones, and D.V.D. Streaten 2007, Monitoring and screening plant populations with combined thermal and chlorophyll fluorescence imaging. J Exp Bot 58:773-784. doi:10.1093/jxb/erl257 10.1093/jxb/erl25717189594
4
Chandler P.M., and M. Robertson 1999, Gibberellin dose-response curves and the characterization of dwarf mutants of barley. Plant Physiol 120:623-32. doi:10.1104/pp.120.2.623 10.1104/pp.120.2.62310364415PMC59302
5
Cocco C., J.L. Andriolo, L. Erpen, F.L. Cardoso, and G.S. Casagrande 2010, Development and fruit yield of strawberry plants as affected by crown diameter and plantlet growing period. Pesq Agropec Bras Brasilia 45:730-736. doi:10.1590/S0100-204X2010000700014 10.1590/S0100-204X2010000700014
6
Corr B.E., and R.E. Widmer 1987, Gibberellic acid increases flower number in Zantedeschia elliottiana and Z. rehmanii. HortScience 23:1001-1002. doi:10.21273/HORTSCI.22.4.605 10.21273/HORTSCI.22.4.605
7
Csukasi F., S. Osorio, and J.R. Gutierrez 2011, Gibberellin biosynthesis and signalling during development of the strawberry receptacle. New Phytol 191:376-390. doi:10.1111/j.1469-8137.2011.03700.x 10.1111/j.1469-8137.2011.03700.x21443649
8
Deyton D.E., C.E. Sams, and J.C. Cummins 1991, Strawberry growth and photosynthetic responses to paclobutrazol. HortScience 26:1178-1180. doi:10.21273/HORTSCI.26.9.1178 10.21273/HORTSCI.26.9.1178
9
Dill A., H.S. Jung, and T.P. Sun 2001, The DELLA motif is essential for gibberellin-induced degradation of RGA. Proc Natl Acad Sci 98:14162-14167. doi:10.1073/pnas.251534098 10.1073/pnas.25153409811717468PMC61185
10
Durner E.F., E.B. Poling, and J.L. Maas 2002, Recent advances in strawberry plug transplant technology. HortTechnology 12:545-550. doi:10.21273/HORTTECH.12.4.545 10.21273/HORTTECH.12.4.545
11
Fu X., and N.P. Harberd 2003, Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature 421:740-743. doi:10.1038/nature01387 10.1038/nature0138712610625
12
Guleria S., M. Kumar, A. Khan, and R. Kaushik 2021, Plant hormones: Physiological role and health effects. J Microbiol Biotechnol Food Sci 11:1-8. doi:10.15414/jmbfs.1147 10.15414/jmbfs.1147
13
Guttridge C.G., and P.A. Thompson 1964, The effect of gibberellins on growth and flowering of Fragaria and Duchesnea. J Exp Bot 15:631-646. doi:10.1093/jxb/15.3.631 10.1093/jxb/15.3.631
14
Hasse L., M. Pritts, and M. Eames-Sheavly 1989, Growth regulators affect vegetative and reproductive growth in a dayneutral and Junebearing strawberry cultivar. Adv Strawberry Prod 8:45-50.
15
Hazrati S., Z. Tahmasebi-Savestani, S.A.M. Modarres-Sanavy, A. Mokhassi-Bidgoli, and S. Nicola 2016, Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L. Plant Physiol Biochem 106:141-148. doi:10.1016/j.plaphy.2016.04.046 10.1016/j.plaphy.2016.04.04627161580
16
Hisamatsu T., M. Koshioka, N. Oyama, and L.N. Mander 1999, The relationship between endogenous gibberellins and rosetting in Eustoma grandiflorum. J Jpn Soc Hortic Sci 68:527-533. doi:10.2503/jjshs.68.527 10.2503/jjshs.68.527
17
Hwang H.S., H.W. Jeong, H.R. Lee, and S.J. Hwang 2020, Rooting rate and survival rate as affected by humidification period and medium type of 'Maehyang' strawberry on cutting propagation. Protected Hort Plant Fac 29:219-230. (in Korean) doi:10.12791/KSBEC.2020.29.3.219 10.12791/KSBEC.2020.29.3.219
18
Jun H.J., E.H. Jeon, S.I. Kang, and G.H. Bae 2014, Optimum nutrient solution strength for Korean strawberry cultivar 'Daewang' during seedling period. Korean J Hortic Sci Technol 32:812-818. (in Korean) doi:10.7235/hort.2014.14079 10.7235/hort.2014.14079
19
Kang D.I., H.K. Jeong, Y.G. Park, H Wei, J Hu, and B.R. Jeong 2019, Humidification and shading affect growth and developꠓment of cutting propagated 'Maehyang' strawberry (Fragaria × ananassa Duch.) at propagation stage. Protected Hort Plant Fac 28:429-437. (in Korean) doi:10.12791/KSBEC.2019.28.4.429 10.12791/KSBEC.2019.28.4.429
20
Kang H.J., H.J. Song, S.J. Park, Z.H. Kim, and S.W. Lee 2011, Effects of crown diameter on plant growth and fruit yield in strawberry (Fragaria × ananassa Duch.). J Agric Life Sci 45:81-86. (in Korean)
21
Kang J.H., H.M. Kim, H.M. Kim, H.W. Jeong, H.R. Lee, H.S. Hwang, B.R. Jeong, N.J. Kang, and S.J. Hwang 2018, Gibberellin application method and concentration affect to growth, runner, and daughter plant production in 'Maehyang' strawberry during nursery period. Protected Hort Plant Fac 27:407-414. (in Korean) doi:10.12791/KSBEC.2018.27.4.407 10.12791/KSBEC.2018.27.4.407
22
Kim C.S., and Z.S. Kim 2012, Effects of cutting time, auxin treatment, and cutting position on rooting of the green-wood cuttings and growth characteristics of transplanted cuttings in the adult Prunus yedoensis. Korean J Hortic Sci Technol 30:129-136. (in Korean) doi:10.7235/hort.2012.11041 10.7235/hort.2012.11041
23
Kim E.J., C.S. Kim, H.S. Jung, and J.G. Lee 2023, Promotion of growth and development of 'Seolhyang' strawberry (Fragaria × ananassa Duch.) runner plant by gibberellin treatment. Protected Hort Plant Fac 32:295-301. (in Korean). doi:10.12791/KSBEC.2023.32.4.295 10.12791/KSBEC.2023.32.4.295
24
Kim E.J., J.Y. Choi, M.R. Yu, M.Y. Kim, S.H. Lee, and B.H. Lee 2012, Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Korean J Food Sci Technol 44:337-342. (in Korean) 10.9721/KJFST.2012.44.3.337
25
Kim H.M., H.M. Kim, H.W. Jeong, H.R. Lee, B.R. Jeong, N.J. Kang, and S.J. Hwang 2018, Growth and rooting rate of 'Maehyang' strawberry as affected by irrigation method on cutting propagation in summer season. Protected Hort Plant Fac 27:103-110. (in Korean) doi:10.12791/KSBEC.2018.27.2.103 10.12791/KSBEC.2018.27.2.103
26
Kim T.I., W.S. Kim, J.H. Choi, W.S. Jang, and K.S. Seo 1999, Comparison of runner production and growth characteristics among strawberry cultivars. Korean J Hortic Sci Technol 17:111-114. (in Korean)
27
Kong M.R., and Y.S. Lee 2015, Anti-oxidative and anti-aging properties of hot water extracts from fertile and sterile E.arvense stems. J Invest Cosmetol 11:315-324. (in Korean) 10.15810/jic.2015.11.4.005
28
Lee J.E., H.W. Do, D.W. Choi, J.D. Cheung, M.K. Kim, and Y.S. Shin 2013, Runner production as influenced by gibbrrellin treatment in mother plant of strawberry (Fragaria × ananassa Duch.). Korean J Hortic Sci Technol 31(Suppl II):55. (in Korean)
29
Lopez-Galarza S., B. Pascual, J. Algarda, and J.V. Maroto 1989, The influence of winter gibberellic acid applications on earliness, productivity and other parameters of quality in strawberry cultivation (Fragaria × ananassa Duch.) on the spanish mediterranean coast. Acta Hortic 265:217-222. doi:10.17660/ActaHortic.1989.265.32 10.17660/ActaHortic.1989.265.32
30
Ministry of Agriculture, Food and Rural Affairs (MAFRA) 2022, 2021 production amount and index of agriculture and forestry. Available via http://www.mafra.go.kr Accessed 3 April 2023.
31
Momenpour A., T.S. Taghavi, and S. Manochehr 2011, Effects of benzyladenine and gibberellin on runner production and some vegetative traits of three strawberry cultivars. Afr J Agric Res 6:4357-4361.
32
Moon Y.E., Y.H. Kim, C.M. Kim, and S.O. Kho 2003, Effects of foliar application of GA3 on flowering and fruit quality of very early-maturing satsuma mandarin. Korean J Hortic Sci Technol 21:110-113.
33
Pamfil D., and C. Bellini 2011, Auxin control in the formation of adventitious roots. Not Bot Hortic Agrobot Cluj-Napoca 39:307-316. doi:10.15835/nbha3916101 10.15835/nbha3916101
34
Park B.S. 2020, A study on growth and total polyphenols, flavonoid contents and antioxidant activity of buckwheat by gibberellin and silicic acid application. Master Thesis, Kangwon National Univ., Chuncheon, Korea.
35
Pharis R.P., and R.W. King 1985, Gibberellins and reproductive development in seed plants. Ann Rev plant Physiol 36:517-568. doi:10.1146/annurev.pp.36.060185.002505 10.1146/annurev.pp.36.060185.002505
36
Porlingis I.C., and D. Boynton 1961, Growth responses of the strawberry plant, Fragaria chiloensis var. ananassa, to gibberellic acid and to environmental conditions. J Am Soc Hortic Sci 78:261-269.
37
Ruban A.V. 2016, Nonphotochemical chlorophyll fluorescence quenching: mechanism and effectiveness in protecting plants from photodamage. Plant Physiol 170:1903-1916. doi:10.1104/pp.15.01935 10.1104/pp.15.0193526864015PMC4825125
38
Rural Development Administration (RDA) 2017, Standard manual of characteristics investigation for breeding new varieties of strawberries. RDA, Jeonju, Korea.
39
Singleton V.L., and J.A. Rossi 1965, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144-158. doi:10.5344/ajev.1965.16.3.144 10.5344/ajev
40
Symons G.M., C. Davies, Y. Shavrukov, I.B. Dry, J.B. Reid, and M.R. Thomas 2006, Grapes on steroids. Brassinosteroids are involved in grape berry ripening. Plant Physiol 140:150-158. doi:10.1104/pp.105.070706 10.1104/pp.105.07070616361521PMC1326039
41
Takaki T., A. Tomiyasu, M. Matsushima, and T. Suzuki 1989, Seasonal changes of GA-like substances in fruit and current shoots of satsuma mandarin trees. J Jpn Soc Hortic Sci 58:569-573. doi:10.2503/jjshs.58.569 10.2503/jjshs.58.569
42
Thompson P.A. 1969, The effect of applied growth substances on development of the strawberry fruit: II. Interactions of auxins and gibberellins. J Exp Bot 20:629-647. doi:10.1093/jxb/20.3.629 10.1093/jxb/20.3.629
43
Thompson P.A., and C.G. Guttridge 1959, Effect of gibberellic acid on the initiation of flowers and runners in the strawberry. Nature 184:72-73. doi:10.1038/184072a0b 10.1038/184072a0b
44
Uematsu Y. 1998, Principles and practices in strawberry cultivation. Seibundo-shinkosha, Tokyo, Japan, pp 2-44 (in Japanese).
45
Yoshizawa S., T. Hhoriuchi, T. Yoshida, and T. Okuda 1987, Antitumor promoting activity of (-)-epigallocatechin gallate, the main constitutions of tannin green tea. Phytother Res 1:44-47. doi:10.1002/ptr.2650010110 10.1002/ptr.2650010110
46
Zhishen J., T. Mengcheng, and W. Jianming 1999, The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555-559. doi:10.1016/S0308-8146(98)00102-2 10.1016/S0308-8146(98)00102-2
Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Journal of Bio-Environment Control
  • Journal Title(Ko) :생물환경조절학회지
  • Volume : 33
  • No :1
  • Pages :12-21
  • Received Date : 2023-11-06
  • Revised Date : 2023-12-13
  • Accepted Date : 2023-12-14
  • DOI :https://doi.org/10.12791/KSBEC.2024.33.1.012
Journal Informaiton Journal of Bio-Environment Control Journal of Bio-Environment Control
Online Submission
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crossref funder-registry
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close