Journal of Bio-Environment Control ISSN:1229-4675(Print) 2765-3641(Online) 생물환경조절학회지

It was intended to closely examine an effect that a change in the concentration of culture medium had on the potato(Solanum tuberosum L.) plantlet growth in the microponic system so as to mass-produce the virus-free plant of new variety ‘Saebong’ for potato processing. The adjusted concentration of potato culture medium was 0.2, 0.6, 1.0, 1.4, 1.8, and 14.0 dS· m⁻¹. And potato seedling was cut into pieces of 1.5 cm in length, which included 2 growth points and leaves. And each was explanted in glass vial of 50 mL. And experiments were carried out twice for 18 days or 21days. Culture medium of 2ml was put in the container respectively. And 1 mL was added after 10 days. And in terms of cultivation environment, the experiment was carried out at the day length of 16 hours at the temperature of 23 ± 1°C under the white LED light of 40 μmol·m⁻²·s⁻¹. The concentration of culture medium in the experiment I was EC 0.2, 1.0, 14 dS·m⁻¹ and was adjusted to 0.6, 1.0, 1.4, 1.8 dS·m⁻¹ in the experiment II. The results showed that the survival rate of plantlet was 90% at 0.2 dS ² m-1, 100% at 0.6 dS ² m-1, 100% at 1.0 dS ² m-1. 0% at 1.4 dS·m⁻¹, 0% at 1.8 dS·m⁻¹. and 0% at 14.0 dS·m⁻¹ after 7 days. With regard to the explanted potato seedling, in case of the treatment where the electrical conductivity of culture medium was adjusted to 1.0 dS·m⁻¹, root developed 2 days after transplantation. And the plantlet vigorously grew into strong plant that had 7 leaves, length of 5cm, and fresh weight of 0.5 g after 18 days. In case of the treatment where the concentration of culture medium was adjusted to 0.6 dS·m⁻¹, the root plantlets developed 4 days after transplantation. And those grew into plant that had 7 leaves and fresh weight of 0.2 g after 21 days. Therefore, we found that it is effective to control potato culture medium by adjusting its electrical conductivity to 0.6~1.0 dS·m⁻¹ for the mass production of virus-free potato seedling in the microponic system.