All Issue

2019 Vol.28, Issue 4 Preview Page
Germination Characteristics of Medicinal Crop Adenophora triphylla var. japonica Hara as Affected by Seed Disinfection and Light Quality

종자 소독처리와 광질에 따른 약용작물 잔대 종자의 발아특성

Hye Ri Lee1
,
Hyeon Min Kim1
,
Hyeon Woo Jeong1
,
Myung Min Oh5
,
Seung Jae Hwang1234*
이 혜리1
,
김 현민1
,
정 현우1
,
오 명민5
,
황 승재1234*
1Division of Applied Life Science, Graduate School of Gyeongsang National University, Jinju 52828, Korea
2Department of Agricultural Plant Science, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea
3Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Korea
4Reserch Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea
5Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, Korea
1경상대학교 대학원 응용생명과학부
2경상대학교 농업생명과학대학 농업식물과학과
3경상대학교 농업생명과학연구원
4경상대학교 생명과학연구원
5충북대학교 축산·원예·식품공학부 원예학전공
*Corresponding author: hsj@gnu.ac.kr
30 November 2019. pp. 404-410
PDF XML Citation
Abstract

This study was performed to investigate the seed morphological characteristics and dormancy type of Adenophora triphylla var. japonica Hara that high valued medicinal crop and to select the disinfectants and light quality for germination rate improvement. The seed disinfection was carried out using distilled water (control), NaClO 4%, H2O2 4%, and benomyl 500 mg·L-1. The light quality treatments were set to dark condition (control I), fluorescent lamp (control II), LEDs [red, blue, green, and combined RB LEDs (red:blue = 8:2, 6:4, 4:6, 2:8)] with a photoperiod of 12/12 (light/dark) and light intensity 150 ± 10 μmol·m-2·s-1 photosynthetic photon flux density. Although the Adenophora triphylla var. japonica Hara seed was an underdeveloped embryo (E) and seed (S) with an embryo (E):seed (S) ratio of 0.4, it is germinated within 30 days, and seed moisture saturation was reached within 6 hours after immersion. After seed disinfection, the mold incidence rate was significantly inhibited, and the final germination rate was the highest at 87% in the benomyl seed disinfection. The final germination rate was the highest at 92% in the red light, and the mean daily germination was the lowest in the R2B8. Therefore, there is almost no dormancy in the Adenophora triphylla var. japonica Hara seed, and benomyl seed disinfectant and red light were effective in the improvement of germination rate. So it is considered to the high value of use for medicinal crop Adenophora triphylla var. japonica Hara cultivation.

Keywords
benomyl
dormancy
mean daily germination
underdeveloped embryo

본 연구는 고부가가치 약용작물인 잔대(Adenophora triphylla var. japonica Hara)의 종자 형태특성과 휴면 유 형을 조사하고, 발아율 향상을 위한 소독제와 광질을 선 발하기 위해서 수행되었다. 종자 소독은 증류수(대조구), NaClO 4%, H2O2 4%와 benomyl 500mg·L-1를 이용하여 실시하였으며, 광질처리는 암조건(control I), 형광등 (control II), LEDs[red, blue, green, RB LEDs(red:blue = 8:2, 6:4, 4:6, 2:8)]를 광주기 12/12(light/dark), 광도 150μmol·m-2·s-1 photosynthetic photon flux density로 설정 하였다. 잔대 종자는 emryo (E):seed (S) ratio가 0.4로 미 숙배 종자지만 30일 이내에 발아가 되며, 침지 6시간 만 에 포화상태에 도달하였다. 종자 소독 후 benomyl 처리에 서 곰팡이 발생이 유의적으로 억제되었으며, 최종 발아 율이 87%로 가장 높았다. 적색광에서 최종 발아율이 92%로 가장 높았으며, 일일평균발아수는 R2B8에서 가 장 적었다. 따라서 잔대 종자는 휴면이 거의 없으며, benomyl 소독제와 적색광이 발아율 향상에 효과적이여, 향후 약용작물 잔대 재배에 이용가치가 높을 것이라 판 단된다.

키워드
미숙배
베노밀
일일평균발아수
휴면
References
1
Baskin, C.C., and J.M. Baskin. 2014. Seeds: ecology, biogeography, evolution of dormancy and germination. Academic Press, San Diego, USA.
2
Baskin, J.M., and C.C. Baskin. 2004. A classification system for seed dormancy. Seed Sci. Res. 14:1-16. 10.1079/SSR2003150
10.1079/SSR2003150
3
Borthwick, H.A., S.B. Hendricks, E.H. Toole, and V.K. Toole. 1954. Action of light on lettuce seed germination. Bot. Gaz. 115:205-225. 10.1086/335817
10.1086/335817
4
Carpita, N.C. and N.W. Nabors. 1976. Effects of 35oC heat treatments on photosensitive 'Grand Rapid' lettuce seed germination. Plant Physiol. 57:612-616. 10.1104/pp.57.4.61216659537PMC542083
10.1104/pp.57.4.61216659537PMC542083
5
Cho, G.Y., D.M. Son, J.M. Kim, B.S. Seo, S.Y. Yang, J.H. Bae, and B.G. Heo. 2008. Effect of LED as light quality on the germination, growth and physiological activities of broccoli sprouts. J. Bio-Environ. Con. 13:81-89. (in Korean)
6
Cho, J.S., J.H. Jeong, S.Y. Kim and C.H. Lee. 2014. Temperature, light and chemical treatment promoting seed germination of Meterostachys sikokiana (Makino) Nakai. J. Flower Res. 22:54-59. (in Korean) 10.11623/frj.2014.22.2.3
10.11623/frj.2014.22.2.3
7
Choi, H., S.Y. Lee, Y.H. Rhie, J.H. Lee, S.Y. Kim, and K.C. Lee. 2018. Seed dormancy type and germination characteristics in Tiarella polyphylla D. Don native to Korea. Korean J. Plant Res. 31:363-371. (in Korean)
8
Choi, H.J., S.H. Kim, H.T. Oh, M.J. Chung, C.B. Cui, and S.S Ham. 2008. Effects of Adenophora triphylla ethylacetate extract on mRNA levels of antioxidant enzymes in human HepG2 cells. Korean J. Soc. Food Sci. Nutr. 37:1238-1243. (in Korean) 10.3746/jkfn.2008.37.10.1238
10.3746/jkfn.2008.37.10.1238
9
Chung, M.J., S. Lee, Y.I. Park, and K.H. Kwon. 2016a. Antioxidative and neuroprotective effects of extract and fractions from Adenophora triphylla. Korean J. Soc. Food Sci. Nutr. 45:1580-1588. (in Korean) 10.3746/jkfn.2016.45.11.1580
10.3746/jkfn.2016.45.11.1580
10
Chung, M.J., S. Lee, Y.I. Park, and K.H. Kwon. 2016b. Neuroprotective effects of phytosterols and flavonoids from Cirsium setidens and Aster scaber in human brain neuroblastoma SK-N-SH cells. Life Sci. 148:173-182. (in Korean) 10.1016/j.lfs.2016.02.03526874034
10.1016/j.lfs.2016.02.03526874034
11
Delaquis, P.J., P.L. Sholberg, and K. Stanich. 1999. Disinfection of mung bean seed with gaseous acetic acid. J. Food Prot. 62:953-957. 10.4315/0362-028X-62.8.95310456753
10.4315/0362-028X-62.8.95310456753
12
Edwards, T.I. 1934. Relations of germinating soybeans to temperature and length of incubation time. Plant Physiology 9:1-30. 10.1104/pp.9.1.116652864PMC439825
10.1104/pp.9.1.116652864PMC439825
13
Fett. W.F. 2002. Reduction of Escherichia coli O157:H7 and Salmonella spp. on laboratory inoculated mung bean seed by chlorine treatment. J. Food Protection 65:848-852. 10.4315/0362-028X-65.5.84812030299
10.4315/0362-028X-65.5.84812030299
14
Gitaitis, R. and R. Walcott. 2007. The epidemiology and management of seedborne bacterial diseases. Annu. Rev. Phytopathol. 45:371-397. 10.1146/annurev.phyto.45.062806.09432117474875
10.1146/annurev.phyto.45.062806.09432117474875
15
Gordon, A.G. 1971. The germination resistance test-A new test for measuring germination quality of cereals. J. Canadian Plant Sci. 51:181-183. 10.4141/cjps71-036
10.4141/cjps71-036
16
Gorovoi, P.G., G.I. Ponomarchuk, and L.I. Strigina. 1971. Chemotaxonomic study of Codonopsis (Family Campanulaceae) and its related genera. Bio. chem. System. Ecol. 23:809-812.
17
Ham, Y.A., H.J. Choi, M.J. Chung, and S.S. Ham. 2009a. Component analysis and antioxidant activity of Adenophora triphylla. Korean J. Soc. Food Sci. Nutr. 38: 274-379.(in Korean) 10.3746/jkfn.2009.38.3.274
10.3746/jkfn.2009.38.3.274
18
Ham, Y.A., H.J. Choi, M.J. Chung, and S.S. Ham. 2009b. Antimutagenic and antitumor effects of Adenophora triphylla extracts. Korean J. Soc. Food Sci. Nutr. 38: 25-31. (in Korean) 10.3746/jkfn.2009.38.1.025
10.3746/jkfn.2009.38.1.025
19
Hopkins, W.G. 1999. Introduction to plant physiology. John Wiley &Sons, Inc., USA.
20
Hwang, H.J., and G.W. Choi. 2008. Improvement of in virto seed germination in chicory (Cichorium intybus L.) and garland chrysanthemum (Chrysanthemum coronarium L.). J. Bio-Environ. Con. 17:297-305. (in Korean)
21
Hwang, H.J., J.M. Lee, S.Y. Kim, and G.W. Choi. 2008. Seed germination in lettuce affected by light quality and plant growth regulators. J. Bio-Environ. Con. 17:51-59. (in Korean)
22
Ji, Y.U., B.C. Moon, A.Y. Lee, J.M. Chun, B.K. Choo, and H.K. Kim. 2010. Molecular phylogenetic position of Adenophora racemosa, an endemic species in Korea. Korean J. Med. Crop Sci. 18:379-388. (in Korean)
23
Ji, Y.U., B.C. Moon, A.Y. Lee, T.S. Yoon, H.K. Song, B.K. Choo, and H.K. Kim. 2009. A study on classification and ordination of Adenophora racemosa population. Korean J. Environ. Res. Tech. 12:86-98. (in Korean)
24
Kendrick, R.E., and G.H.M. Kronenberg. 1994. Photomorphogensis in plants. Kluwer Academic, Dordrecht, The Netherlands.
25
Kim, I.D., and S.D. Kim. 2001. Changes in quality of soybean sprouts grown by ozone water treatment during storage. Korean J. Postharvest Sci. Tech. 8:279-384. (in Korean)
26
Kim, I.D., M.J. Park, J.W. Cho, S.S. Soe, M.K. Kim, J.B. Lee, S.K. Lee, and S.D. Kim. 2001. Effect of ozone treatment on the quality of soybean sprouts. Korean J. Food Preserv. 5:177-185. (in Korean)
27
Kim, J.H. 2007. The study on the nutrients and function of Adenophora triphylla var. japonica Hara. Master Thesis. Yeungnam Univ.. (in Korean)
28
Kwack, B.H., and H. Kang. 1985. Effects of specific light qualities on the seed germination of Amaranthus hypochondriacus. Korean J. Soc. Hort. Sci. 26:158-162.
29
Lee, J.Y., J.H. Lee, G.Y. Ki, S.T. Kim, and T.H. Han. 2011. Improvement of seed germination in Rosa rugosa. Korean J. Hortic. Sci. Technol. 29:352-357. (in Korean) 10.5763/kjsm.2011.29.1.9
10.5763/kjsm.2011.29.1.9
30
Martin, A. 1946. The comparative internal morphology of seeds. The Amer. Midl. Nat. 36:513-660. 10.2307/2421457
10.2307/2421457
31
Moon, J.S., H.M. Kim, D.C. Choi, Y.K. Hong, M.H. Sung, Y.J. Jang, B.R. Go, N.K. Oh, and Y.G. Choi. 2003. Disinfection of seed borne black leg disease (Phoma Wasabiae) in wasabi (Wasabia japonica Mutsum). J. Bio-Environ. Con. 12:180-183. (in Korean)
32
Nikolaeva, M.G. 1999. Pattern of seeds dormancy and germination as related to plant phylogeny and ecological and geographical conditions of their habitats. J. Russ. Plant Physiol. 46:369-373.
33
Park, D.S. 2010. The edible wild vegetable containing biological active substances. euliiglobal. Korea. pp 231-234.
34
Park, E.J., J.H. Kwon, and Y.K. Lee. 2009. Germination rate and microbial safety during cultivation of disinfected seeds. Korean J. Food Preserv. 16:292-298. (in Korean)
35
Park, K.J., J.H. Lim, J.H. Kim, J.W. Jung, J.H. Jo, and S.W. Jung. 2007. Reduction of microbial load on radish (Raphanus sativus L.) seeds by aqueous chlorine dioxide and hot water treatments. Korean J. Food Preserv. 14:487-491. (in Korean)
36
Vandelook, F., N. Bolle, and J.A. V. Assche. 2007. Seed dormancy and germination of the European Chaerophyllum temulum (Apiaceae), a member of a trans-atlantic genus. Ann. Bot. 100:233-139. 10.1093/aob/mcm09017556382PMC2735313
10.1093/aob/mcm09017556382PMC2735313
Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Protected Horticulture and Plant Factory
  • Journal Title(Ko) :시설원예ㆍ식물공장
  • Volume : 28
  • No :4
  • Pages :404-410
  • Received Date : 2019-08-22
  • Revised Date : 2019-10-02
  • Accepted Date : 2019-10-08
  • DOI :https://doi.org/10.12791/KSBEC.2019.28.4.404
Journal Informaiton Journal of Bio-Environment Control Journal of Bio-Environment Control
Online Submission
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crossref funder-registry
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close