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2020 Vol.29, Issue 4 Preview Page

Original Articles

Effect of Plasma-activated Water Process on the Growth and Functional Substance Content of Lettuce during the Cultivation Period in a Deep Flow Technique System

담액수경재배 시스템에서 플라즈마수 처리가 상추의 생육 및 페놀류 함량에 미치는 영향

Seung Won Noh1†
,
Jong Seok Park2†
,
Sung Jin Kim1
,
Dae-Woong Kim3
,
Woo Seok Kang4*
노 승원1†
,
박 종석2†
,
김 성진1
,
김 대웅3
,
강 우석4*
1Graduate Student, Department of Horticultural Science, Chungnam National University, Daejeon 34134, Korea
2Professor, Department of Horticultural Science, Chungnam National University, Daejeon 34134, Korea
3Senior Researcher, Department of Plasma Engineering, Korea Institute of Machinery & Materials, Daejeon 34103, Korea
4Principal Researcher, Department of Plasma Engineering, Korea Institute of Machinery & Materials, Daejeon 34103, Korea
1충남대학교 농업생명과학대학 원예학과 대학원생
2남대학교 농업생명과학대학 원예학과 교수
3한국기계연구원 플라즈마연구실 선임연구원
4한국기계연구원 플라즈마연구실 책임연구원
*Corresponding author: kang@kimm.re.kr
†These authors contributed equally to this work
†These authors contributed equally to this work
30 October 2020. pp. 464-472
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Abstract

We suggest a hydroponic cultivation system combined with a plasma generator to investigate the changes in the growth and functional substance content of lettuces during the cultivation period. Lettuce seedlings of uniform size were planted in semi-DFT after seeding for 3 weeks, and the plasma-activated water was intermittently operated for 1 hour at an 8 hours cycle for 4 weeks. Lettuces grew with or without plasma-activated water with the nutrient solution in hydroponics culture systems. Among the reactive oxygen species generated during plasma-activated water treatment, brown spots and necrosis appeared in the individuals closer to the plasma generating device due to O3, and there was no significant difference in the growth parameters. While the rutin and total phenolic content of the lettuce shoot grown in the nutrient solution were higher than that of the plasma-activated water, epicatechin contents in plasma-activated water were significantly greater than the nutrient solution. However, in the roots, all kinds of secondary metabolites measured in this work, rutin, epicatechin, quercetin, and total phenolic contents, were significantly higher in the plasma-activated water than the control. These results were indicated that the growth of lettuce was decreased due to the reactive oxygen species such as ozone in the plasma-activated water, but the secondary metabolites in the root zone increased significantly. It has needed to use this technology for the cultivation of root vegetables with the modified plasma- activated water systems to increase secondary metabolite in the roots.

Keywords
hydroponics
nitrate(NO3-)
plasma-activated water
reactive oxygen species
secondary metabolites

본 연구에서는 플라즈마 발생장치를 수경재배 시스템과 결합하여 재배 기간 동안 처리 시 상추의 생육 및 기능성 물질 함량 변화를 살펴보기 위해 실시하였다. 3주 동안 육묘하여 균일한 크기의 상추 묘를 semi-DFT에 정식하였으며, 플라즈마 공정 장치를 결합하여 4주 동안 8시간 주기로 1시간씩 수중에서 간헐적으로 작동시켰다. 양액(대조구), 플라즈마 활성수(4.2kV, 5.7kV)를 사용하여 온실에서 재배하였으며 이후 수확하여 생육조사 및 기능성 물질 분석을 실시하였다. 플라즈마 활성수 처리 기간 동안 발생되는 활성산소종 중에서 O3로 인하여 플라즈마 발생 장치에 근접한 개체일수록 갈색 반점 및 괴사현상이 나타났으며, 생육조사를 실시한 결과 유의적 차이가 나타나지 않았다. 기능성 물질 분석 결과 상추 지상부의 rutin과 총 페놀 함량은 플라즈마수보다 높았지만, epicatechin의 경우 플라즈마수 처리에서 함량이 더 많았다. 근권부에서 측정된 이차대사산물인 rutin, epicatechin, quercetin 및 총 페놀 함량은 대조구보다 플라즈마수 처리구에서 유의하게 높았다. 이러한 결과는 플라즈마수 처리 시간동안 수중에 오존과 같은 활성산소종으로 인해 지상부 생육이 잘 이루어지지 못했으나, 근권 영역에서는 이차대사산물이 크게 증가하였다. 향후 간헐적인 플라즈마 활성수 생성에 따른 생리 장해를 극복하고 뿌리채소의 수경재배 시스템에 적용하여 이차대사산물을 증가시키기 위한 본 기술의 도입이 필요하다.

키워드
이차대사산물
수경재배
질산염
플라즈마수
활성산소종
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Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Protected Horticulture and Plant Factory
  • Journal Title(Ko) :시설원예ㆍ식물공장
  • Volume : 29
  • No :4
  • Pages :464-472
  • Received Date : 2020-09-25
  • Revised Date : 2020-10-16
  • Accepted Date : 2020-10-17
  • DOI :https://doi.org/10.12791/KSBEC.2020.29.4.464
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