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2022 Vol.31, Issue 2 Preview Page

Original Articles

Effects of Different Natural Extracts and Plant Growth Regulators on Plant Regeneration and Callus Induction from Pseudobulbs Explants through in vitro Seed Germination of Endangered Orchid Bulbophyllum auricomum Lindl.

멸종 위기에 처한 Bulbophyllum auricomum Lindl. orchid의 시험관 내 종자 발아를 통한 구근 절편체의 식물 재생 및 캘러스 유도에 대한 천연 추출물 및 식물 성장 조절제(PGR)의 효과

Win Theingi Aung1
,
Keuk Soo Bang2*
,
Seo A Yoon3
,
Baul Ko4
,
Jong Hyang Bae5*
땡기 아웅1
,
방 극수2*
,
윤 서아3
,
고 바울4
,
배 종향5*
1Doctor’s Degree, Department of Lifestyle Medicine, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan 54596, Korea
2Professor, Department of Oriental Medicine Resources, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan 54596, Korea
3Doctor’s Degree, Institute of Life Science and Natural Resources, Wonkwang University, Iksan 54538, Korea
4Post-Doctoral Researcher, Urban Agriculture Division. National Institute of Horticultural & Herbal Science, RDA, Wanju 55365, Korea
5Professor, Department of Horticulture Industry, Wonkwang University, Iksan 54538, Korea
1전북대학교 환경생명자원과학대학 라이프스타일 의학과 박사과정
2전북대학교 환경생명자원과학대학 한약자원학과 교수
3전북대학교 환경생명자원과학대학 한약자원학과 교수
4국립원예특작과학원 도시농업과 박사 후 연구원
5원광대학교 원예산업학과 교수
*Corresponding Author
30 April 2022. pp. 133-141
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Abstract

Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L-1 of BAP and 2.0 mg·L-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L-1 of banana and 20 g·L-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L-1 of coconut water) and plant growth regulators (2.0 mg·L-1 of BAP and 1.0 mg·L-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively.

Keywords
artificial self-pollination
tissue culture
natural extract
plant growth regulator

Bulbophyllum auricomum Lindl.은 희귀 난초이며 매력적인 향기가 지닌 꽃이 피어난다. 본 연구는 미세 증식을 위한 조직 배양 방법을 조사하였다. 인공 자가 수분으로부터 파생된 캡슐은 MS 기본 배지에서 최상의 종자 발아를 위해 얻어진다. 식물 성장 조절제(1.0 mg/L의 BAP 및 2.0 mg/L의 NAA)는 원괴체 유래 유사구근 절편체의 캘러스 유도에 영향을 받았다. 캘러스 계대배양을 위해 10가지 처리 배지에서 다른 천연물 추출물을 테스트하였다. 이 중 150ml/L 코코넛 워터를 첨가한 MS 배지는 일반적으로 1개월 및 2개월에 생중량(1.75 ± 0.08) 및 (3.01 ± 0.20) 캘러스 증식 및 PLB 유도에 효과가 있었고, 그 다음으로 바나나 30g/L와 감자 추출물 20g/L의 MS 조합이 뒤를 이었다. 식물 생장 조절제와 천연 추출물을 함께 첨가한 MS배지와 천연 추출물만 첨가한 MS 배지를 비교 연구한 결과, 천연 추출물(코코넛 워터 150ml/L)과 식물 생장 조절제(BAP 2.0 mg/L 및 NAA 1.0 mg/L)를 혼합하여 MS 배지에 첨가한 결과 ​배양 1개월 및 2개월 후에 각각 가장 높은 새싹 재생수로 (3.37 ± 0.17) 및 (6.41 ± 0.68)을 얻었다.

키워드
인공자가수분
조직배양
천연추출물
식물생장조절제
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Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Journal of Bio-Environment Control
  • Journal Title(Ko) :생물환경조절학회지
  • Volume : 31
  • No :2
  • Pages :133-141
  • Received Date : 2022-04-04
  • Revised Date : 2022-04-27
  • Accepted Date : 2022-04-28
  • DOI :https://doi.org/10.12791/KSBEC.2022.31.2.133
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