All Issue

2015 Vol.24, Issue 1 Preview Page
Influence of Short-term Application of Abscisic Acid in Nutrient Solution on Growth and Drought Tolerance of Tomato Seedlings

토마토 육묘과정에서 단기간 ABA처리가 묘소질과 건조내성에 미치는 영향

Il-Seop Kim1*
,
Ngoc-Thang Vu1
,
Hoang-Tung Vo2
,
Ki-Young Choi3
,
Young Shik Kim4
김 일섭1*
,
넉 탕부1
,
후 앙텅부2
,
최 기영3
,
김 영식4
1Department of Horticulture, Kangwon National University, Chuncheon 200-701. Korea
2Department of Medical Biotechnology, Kangwon National University, Chuncheon 200-701, Korea
3Department of Controlled Agriculture, Kangwon National University, Chuncheon. Korea
4Department of Plant and Food Sciences, Sangmyung Univ.Cheonan 330-720, Korea
1강원대학교 원예학과
2강원대학교 의생명학과
3강원대학교 시설농업학과
4상명대학교 식물식품학과
*Corresponding Author : kimilsop@kangwon.ac.kr
31 January 2015. pp. 13-20
PDF XML Citation
Abstract

This study was conducted to evaluate influence of short-term application of abscisic acid (ABA) in nutrient solution on growth and drought tolerance of tomato seedlings. The treatments included four ABA concentrations (0.5, 1, 2, 3mg·L−1) and control (non-treatment) were applied to the nutrient solution in a hydroponic system. On the 5th and 10th day after growing in the nutrient solution containing ABA, seedlings were transferred to -5 bars of PEG- 8000 in a growth chamber to induce water stress. Except for stem diameter and fresh and dry weight of root, there were no statistical differences in other growth parameters among control, 0.5 and 1mg·L−1 of ABA treatments. Seedlings growths were strongly inhibited in nutrient solution containing 2 and 3mg·L−1 of ABA. The root growth such as fresh and dry weigh of root, total root surface area, and average root diameter was slightly enhanced in 1mg·L−1 of ABA treatment. The elevation of ABA concentrations in nutrient solution resulted in the decrease in transpiration rate and increase in stomatal diffusive resistance and leaf temperature of tomato seedlings. The initiations of seedling wilting after treating in -5 bars of PEG were delayed from 10 hrs in control to 30 hrs in ABA applied treatments. Additionally, the high percentages of recovered seedlings were observed in 0.5 and 1mg·L−1 of ABA treatments after re-irrigation. Therefore, short-term application of 1mg·L−1 of ABA in the nutrient solution stimulated the root growth and drought tolerance of tomato seedlings by delaying the start time of wilting point and enhancing the recovery after re-irrigation.

Keywords
hydroponic system
root morphology
stomatal diffusive resistance
transpiration rate
water stress

본 실험은 단기간 ABA처리가 토마토 묘의 생장과 증산율, 기공 저항성 및 건조 내성에 미치는 영향 을 검토하기 위하여 수행되었다. 실험은 25일간 플러그 트레이에서 육묘한 토마토 묘를 간이 수경재배 키트에 이식하여 양액 육묘하면서 ABA처리 효과와 건조 내성을 검토하였다. 배양액에 ABA를 0.5, 1, 2, 및 3mg·L−1 의 농도로 첨가한 4개의 처리구와 무처리구를 설계하여 5일과 10일간 양액육묘한 뒤 묘소질, 엽온, 증산율, 기공확산 저항성을 측정하였다. 건조 내성을 검토하기 위한 수분 스트레스 처리는 PEG 8,000을 이용하여 -5bar로 조정한 고삼투압 용액에 ABA처리 직후의 묘를 이식한 뒤, 묘의 위조 정도를 조사하였다. 저농도(0.5와 1mg·L−1) 의 ABA처리구에서 묘소질은 경경을 제외하고 대부분의 생육에서 통계적 유의차는 나타나지 않았으나, 2와 3mg·L−1의 농도에서는 지상부의 생장이 억제되었다. 근권부의 생장은 1mg·L−1의 농도처리에서만 뿌리의 건물 중과 생체중, 전표면적, 근장, 근경, root tip수 모두가 유의적으로 증가하였으며, 그 외의 처리농도에서는 일부 의 생육지표를 제외하고는 유의적 차이가 나타나지 않았다. ABA처리 농도가 증가함에 따라 기공확산 저항성 은 증가하고 증산율은 감소하는 경향을 보였다. 또, ABA처리는 묘의 건조 내성을 증가시켜 ABA가 첨가된 배 양액에서 5일 또는 10일간 육묘한 묘를 -5bar 용액에 치상하였을 경우 대조구는 치상후 10시간 후부터 묘의 위조가 시작되어 20시간 후에는 모든 개체가 위조하였으나, ABA처리구는 치상 30시간 후부터 위조가 시작되 어 50시간이 경과해서야 모든 개체가 위조되었다. 또, 수분 스트레스처리로 위조된 묘를 재관수하였을 경우 ABA 0.5와 1mg·L−1처리구는 100%, 그 이상 농도 처리구에서도 50%이상 회복되었으나, 무처리구의 경우는 전개체가 고사하였다. 이상의 결과 토마토 육묘과정에서 저농도의 ABA처리를 통한 근권부의 생장 촉진과 건 조 내성 증진 가능성이 시사되었으나, 상업적 활용을 위해서는 추가적인 검토가 필요할 것으로 판단된다.

키워드
수경재배
뿌리형태
기공확산 저항
증산율
수분 스트레스
References

REFERENCES

1
AA Abou-Mandour and W Hartung, Zeischrift fur Pflanzenphysiolgie, The effect of abscisic acid on growth and development of intact seedlings, root and callus cultures, and stem and root segments of Phaseoluscoccineus, 100; 25-33 (1980)
2
AAC Alves and TL Setter, Crop Sci, Response of cassava to water deficit: Leaf area growth and abscisic acid, 40; 131-137 (2000)
10.2135/cropsci2000.401131x
3
JL Arsenault, S Pouleur, C Messier and R Guay, HortScience, WinRHIZO, a root-measuring system with a unique overlap correction method, 30, 906 (1995)
4
RN Arteca, D Tsia and C Schlagnhaufer, HortScience, Abscisic acid effects on photosynthesis and transpiration in geranium cuttings, 20; 370-372 (1985)
10.3109/00365528509091666
5
I Bakhsh, I Awan, M Sadiq, M Niamatullash, KU Zaman and M Aftab, J. Anim. Plant Sci, Effect of plant growth regulator application at different growth stages on the economical yield potential of coarse rice (Oriza sativa L.), 21; 612-616 (2011)
6
NL Biddington and AS Dearman, Plant Growth Regul, The effect of abscisic acid on root and shoot growth of cauliflower plants, 1; 15-24 (1982)
10.1007/bf00024218
7
C Borel, T Simonneau, D This and F Tardieu, Austral. J. Plant. Physiol, Stomatal conductance and ABA concentration in the xylem sap of barley lines of contrasting genetic origins, 24; 607-615 (1997)
10.1034/j.1399-3054.1994.900324.x
8
RN Carrow, Crop Sci, Drought avoidance characteristics of diverse tall fescue cultivars, 36; 371-377 (1996)
9
RA Creelman, HS Mason, RJ Bensen, JS Boyer and JE Mullet, Plant Physiol, Water deficit and abscisic acid cause differential inhibition of shoot versus root growth in soybean seedlings, 92; 205-214 (1990)
10.1104/pp.92.1.20516667248PMC1062271
10
U Farooq and A Bano, Pakistan J. Bot, Effects of abscisic acid and chlorocholine chloride on nodulation and biochemical content of Vignaradiata L, 38; 1511-1518 (2006)
11
PJ Franks and GD Farquhar, Plant Physiol, The effect of exogenous abscisic acid on stomatal development, stomatal mechanics, and leaf gas exchange in Tradescantiavirginiana, 125; 935-942 (2001)
10.1104/pp.125.2.93511161050PMC64894
12
DH Gaither, DH Lutz and LE Forrenc, Plant Physiol, Abscisic acid stimulates elongation of excised pea root tips, 55; 948-949 (1975)
10.1104/pp.55.5.94816659198PMC541740
13
S Iqbal, A Bano and N Ilyas, J. Agric. Res, Drought and abscisic acid (ABA) induced changes in protein and pigment contents of four wheat (Triticumaestivum L.) accessions, 48; 1-13 (2010)
14
F Jiang and W Hartung, J. Exp. Bot, Long-distance signaling of abscisic acid (ABA): the factors regulating the intensity of the ABA signal, 59; 37-43 (2008)
15
RJ Jones and TA Mansfield, Physiol. Plant, Effect of abscisic and its esters on stomatal aperture and the transpiration ratio, 26; 321-327 (1972)
10.1111/j.1399-3054.1972.tb01117.x
16
IS Kim, CH Zhang, HM Kang and B Mackay, Hort. Environ. Biotechnol, Control of stretching cucumber and tomato plug seedlings using supplemental light, 49; 287-292 (2008)
10.1007/s13580-014-0021-x
17
DI Leskovar and DJ Cabtliffe, J. Amer. Soc. Hort. Sci, Pepper seedling growth response to drought stress and exogenous abscisic acid, 117; 389-393 (1992)
18
J Li, XQ Wang, MB Watson and SM Assman, Science, Regulation of abiotic acid-induced stomatal closure and anion channels by guard cell AAPK kinase, 287; 300-303 (2000)
10.1126/science.287.5451.30010634783
19
XJ Li, MF Yang, H Chen, LQ Qu, F Chen and SH Shen, Biochim. Biophys. Acta, Abscisic acid pretreatment enhances salt tolerance of rice seedlings: Proteomic evidence, 1804; 924-940 (2010)
20
Y Liao, JS Zhang, S Chen and W Zhang, J. Intergr. Plant Biol, Role of soybean GmbZIP132 under abscisic acid and salt stresses, 50; 221-230 (2008)
10.1111/j.1744-7909.2007.00593.x
21
M Ludewig, K Doerffling and H Seifert, Planta, Abscisic acid and water transport in sunflowers, 175; 325-333 (1988)
10.1007/bf0039633724221869
22
TA Mansfield and RJ Jones, Planta, Effect of abscisic acid on potassium uptake and starch content of stomatal guard cells, 101; 147-158 (1971)
10.1007/bf0038762524488344
23
CJ Mittelheuser and RFM Van Stevenninck, Nature, Stomatal closure and inhibition of transpiration induced by (R.S)-abscisic acid, 21; 281-282 (1969)
24
R Munns and GR Cramer, Plant Soil, Is coordination of leaf and root growth mediated by abscisic acid? Opinion, 185; 33-49 (1996)
25
PE Pilet, Physiol Veg, The effect of abscisic acid on aseptically cultured maize roots, 21; 495-500 (1983)
26
PE Pilet and JE Rebeaud, Plant Sci. Letts, Effect of abscisic acid on growth and indolyl-3-acetic acid levels in maize roots, 31; 117-122 (1983)
10.1016/0304-4211(83)90047-0
27
PE Pilet and PW Barlow, Plant Growth Regul, The role of abscisic in root growth and gravireaction: A critical review, 6; 217-265 (1987)
10.1007/bf00024506
28
LR Rajasekaran and TJ Blake, J. Plant Growth Regul, New plant growth regulators protect photosynthesis and enhance growth under drought of jack pine seedlings, 18; 175-181 (1999)
10.1007/pl0000706710688707
29
JM Robertson, KT Hubick, EC Yeung and DM Reid, J. Exp. Bot, Developmental responses to drought and abscisic acid in sunflower roots. I. Root growth, apical anatomy, and osmotic adjustment, 41; 325-327 (1990)
10.1093/jxb/41.3.325
30
JR Seiler and JD Johnson, For. Sci, Physiological and morphological responses of three half-sib families of loblolly pine to water-stress conditioning, 34; 487-495 (1988)
31
RE Sharp, Y Wu, GS Voetberg, IN Saab and ME LeNoble, J. Exp. Bot, Confirmation that abscisic acid accumulation is required for maize primary root elongation at low water potentials, 45; 1743-1751 (1994)
32
H Takahashi, K Koshio and Y Ota, J. Jpn. Soc. Hort. Sci, Effects of ABA application to the culture solution on the growth, water relations and temperature stress in tomato plants, 62; 389-397 (1993)
33
N Waterland, JJ Finer and ML Jones, HortTechnology, Abscisicacid applications decrease stomatal conductance and delay wilting in drought-stressed chrysanthemums, 20; 896-901 (2010)
34
S Watts, JL Rodriguez, E Evans and WJ Davies, Ann. Bot, Root and shoot growth of plants treated with abscisic acid, 47; 595-602 (1981)
35
T Yamaguchi and HE Street, Ann. Bot, Stimulation of growth of excised cultured roots of soya bean by abscisic acid, 41; 1129-1133 (1977)
36
J Zhang, X Zhan and J Liang, New Phytol, Exudation rate and hydraulic conductivity of maize roots are enhanced by soil drying and abscisic acid treatment, 131; 329-336 (1995)
10.1111/j.1469-8137.1995.tb03068.x
Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Protected Horticulture and Plant Factory
  • Journal Title(Ko) :시설원예ㆍ식물공장
  • Volume : 24
  • No :1
  • Pages :13-20
  • Received Date : 2015-01-19
  • Revised Date : 2015-02-02
  • Accepted Date : 2015-02-10
  • DOI :https://doi.org/10.12791/KSBEC.2015.24.1.013
Journal Informaiton Journal of Bio-Environment Control Journal of Bio-Environment Control
Online Submission
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crossref funder-registry
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close