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Growth Characteristics of Lettuce under Different Frequency of Pulse Lighting and RGB Ratio of LEDs

LED의 간헐조명과 RGB 비율에 따른 상추의 품종별 생육 특성

Sungjin Kim1
,
Gwonjeong Bok1
,
Gongin Lee2
,
Jongseok Park1*
김 성진1
,
복 권정1
,
이 공인2
,
박 종석1*
1Department of Horticultural Sciences, Chungnam National University, Daejeon 34134, Korea
2Department of Agricultural Engineering, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea
1충남대학교 농업생명과학대학 원예학과
2국립농업과학원 농업공학부
*Corresponding author: jongseok@cnu.ac.kr
30 April 2017. pp. 123-132
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Abstract

This study was aimed to investigate the effect of 1)irradiation with several different ratios using red, green, and blue LEDs and 2)various pulsed light irradiation with 50% duty ratio using red and blue LEDs on the growth and morphogenesis of three lettuce cultivars (Lactuca sativar L. cv. ‘Jukchukmeon’, ‘Lolo Rosa’, and ‘Grand Rapid’) in hydroponics culture system for 4 weeks after transplanting. Seeds were sown in rock-wool plug trays and they were placed in a culture room which was controlled at 23±1°C/18±1°C temperature and 50-60/70-85% for day and night, respectively, during cultivation period. Irradiated RGB ratios with LEDs were 6:3:1, 5:2.5:2.5, 3:3:4, 2:2:6, and 1:1:8 with 110±3μmol·m -2·s-1 PPFD on the surface of cultivation bed. The frequencies of pulsed lighting was 50, 100, 500, 1,000, 5,000, 10,000, 25,000Hz (20, 10, 0.1, 0.04 ms) with red and blue LEDs and 50% duty ratio. At the RGB ratio of 6:3:1, the average fresh weight of ‘Jukchukmeon’ was significantly higher than that of other RGB treatments, but no significant difference compared to the fluorescent treatment. The average fresh weight at 1:1:8 RGB ratio in ‘Lolo Rosa’ was significantly lower than that of other RGB treatments. Leaf number and fresh weight of ‘Grand Rapid’ were significantly lower in the control and 1:1:8 RGB treatments, compared to the other RGB treatments. As the ratio of blue light increased, leaf length decreased and leaf shape became round in three lettuces. Although there is little change in growth, it could not be found any tendency to affect the growth and morphogenesis of three lettuces caused by increasing or decreasing frequency of pulsed lighting with 50% duty ratio at the 72μmol·m -2·s-1 PPFD.

Keywords
duty ratio
Lactuca sativar
plant growth
PPFD

본 연구는 상추((Lactuca sativar L.)의 3가지 품종에 대해서 RGB LEDs의 각각의 다른 비율과, 듀티비 50% 조건의 RB LEDs를 이용한 여러 가지 주파수를 가지는 펄스광 조사가 상추의 생장과 형태형성에 미치는 영향을 살펴보기 위하여 수행되었다. 파종 후 육묘 기간을 거쳐 유사한 외형을 갖는 묘를 선발하여 재배 룸의 온도와 습도, 23±1°C/50-60%(주간)과 18±1°C/70-85%(야간) 조 건에서 담액수경재배로 4주간 재배하였다. 광합성유효광 량자속밀도(PPFD)는 재배베드 위에서 110±3μmol·m-2·s-1, RGB 비율은 6:3:1, 5:2.5:2.5, 3:3:4, 2:2:6, 1:1:8 이었다. 50% 듀티비를 갖는 펄스광은 RB LEDs로 구성되었고 설정된 주파수는 50, 100, 500, 1,000, 5,000, 10,000, 25,000Hz(20, 10, 0.1, 0.04ms) 이었다. RGB 비율 6:3:1 에서 적축면 상추의 생체중은 다른 RGB 처리구와 비교 하여 유의적으로 높은 값을 보였으나, 대조구인 형광등 처리구와는 유의적 차이가 발생되지 않았다. RGB 비율 1:1:8의 조건에서, 롤로로사는 생체중, 그랜드 래피드는 엽수와 생체중이 다른 RGB 처리구와 비교하여 유의적 으로 낮았다. 청색광의 비율이 증가할수록, 3개 품종 모 두에서 엽장이 감소하면서 엽형이 둥근 형태로 발달하였 다. RB LED로 구성된 LED 광조건 하에서 50% 듀티 비 조건과 처리된 여러 주파수의 증가 또는 감소에 따 른 상추의 생육 및 형태형성에 미치는 경향성을 발견하 기 힘들었다.

키워드
듀티비
Lactuca sativar
식물생장
PPFD
References
1
Carvalho R.F., M. Takaki, and R.A. Azevedo. 2011. Plant pigments: the many face of light perception. Acta Physiol. Plant 33:241-248.
10.1007/s11738-010-0533-7
2
Dong C, L. Shao, G. Liu, M. Wang, H. Liu, B. Xie, B. Li, Y. Fu, and H. Liu. 2015. Photosynthetic characteristics, antioxidant capacity and biomass yield of wheat exposed to intermittent light irradiation with millisecond-scale periods. J. Plant Physiol. 184:28-36.
10.1016/j.jplph.2015.06.01226210319
3
Folta K.M. and S.A. Maruhnich. 2007. Green light: a signal to slow down or stop. J. Exp. Bot. 58:3099-3111.
10.1093/jxb/erm13017630292
4
Folta, K.M. and K.S. Childers. 2008. Light as a growth regulator: controlling plant biology with narrow-bandwidth solid-state lighting systems. HortScience 43:1957-1964
10.21273/HORTSCI.43.7.1957
5
Galen, C., J.J. Rabenold, and E. Liscum. 2007. Functional ecology of a blue light photoreceptor: effects of phototropin1 on root growth enhance drought tolerance in Arabidopsis thaliana. New Pytologist 173:91-99.
10.1111/j.1469-8137.2006.01893.x17176396
6
Goins, G.D., N.C. Yorio, M.M. Sanwo, and C.S. Brown. 1997. Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with and without supplemental blue lighting. J. Exp. Bot. 48:1407-1413.
10.1093/jxb/48.7.140711541074
7
Hashimoto Y, Y. Yi, F. Nyunoya, Y. Anzai, H. Yamazaki, S. Nakayama, and A. Ikeda. 1987. Vegetable growth as affected by on-off light intensity developed for vegetable factory. Acta Hortic. 229:259-264.
10.17660/ActaHortic.1988.229.26
8
Jishi T., R. Matsuda, and K. Fujiwara. 2015 A kinetic model for estimating net photosynthetic rates of cos lettuce leaves under pulsed light. Photosynth Res. 124:107-116.
10.1007/s11120-015-0107-z25736464
9
Johkan M., K. Shoji, F. Goto, S. Hashida, and T. Yoshihara. 2010. Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. HortScience 45:1809-1814.
10.21273/HORTSCI.45.12.1809
10
Johkan M, K. Shoji, F. Goto, S. Hahida, and T. Yoshihara. 2012. Effect of green light wavelength and intensity on photomorphogenesis and photosynthesis in Lactuca sativa. Environ. Exp. Bot. 75:128-133.
10.1016/j.envexpbot.2011.08.010
11
Kim H.H., R. Wheeler, J.C. Sager, N. Yorio, and G. Goins. 2005. Light-emitting diodes as an illumination source for plants: A review of research at Kennedy Space Center. Habitat (Elmsford) 10:71-78.
10.3727/15429660577479123215751143
12
Lee, G.I., H.J. Kim, S.J. Kim, J.W. Lee, and J.S. Park. 2016. Increased growth by LED and accumulation of functional materials by fluorescence lamps in a hydroponics culture system for Angelica gigas. Protected Horticulture and Plant Factory 25:42-48.
10.12791/KSBEC.2016.25.1.42
13
Liu, M., Z. Xu, S. Guo, C. Tang, X. Liu, and X. Jao. 2014. Evaluation of leaf morphology, structure and biochemical substance of balloon flower (Platycodon grandiflorum (Jacq.) A. DC.) plantlets in vitro under different light spectra. Sci. Hortic. 174:112-118.
10.1016/j.scienta.2014.05.006
14
Loo K.H., W.K. Lun, S.C. Tan, Y.M. Lai, and C.K. Tse. 2009. On driving techniques for LEDs: toward a generalized methodology. IEEE Trans. on Power Electron 24:2967-2976.
10.1109/TPEL.2009.2021183
15
Matsuda R, K. Ohashi-Kaneko, K. Fujiwara, and K. Kurata. 2007. Analysis of the relationship between blue-light photon flux density and the photosynthetic properties of spinach (Spinacia oleracea L.) leaves with regard to the acclimation of photosynthesis to growth irradiance. Soil Sci. Plant Nutr. 53:459-465.
10.1111/j.1747-0765.2007.00150.x
16
McCree K.J. 1972. Action spectrum, absorptance and quantum yield of photosynthesis in crop plants. Agric. Meteorol. 9:191-216.
10.1016/0002-1571(71)90022-7
17
Olvera-Gonzalez E, D. Alaniz-Lumbreras, R. IvanovTsonchev, J. Villa-Hernández, C. Olvera-Olvera, E. González-Ramírez, and V. Castaño. 2013a. Intelligent lighting system for plant growth and development. Comput Electron Agric. 92:48-53.
10.1016/j.compag.2012.11.012
18
Olvera-González E, D. Alaniz-Lumbreras, R. IvanovTsonchev, J. Villa-Hernández, I. Rosa-Vargas, I. LópezCruz, and A. Lara-Herrera. 2013b. Chlorophyll fluorescence emission of tomato plants as a response to pulsed light based LEDs. Plant Growth Regul. 69:117-123.
10.1007/s10725-012-9753-8
19
Park, J.E., Y.G. Park, B.R. Jeong, and S.J. Hwang. 2013. Growth of lettuce in closed-type plant production system as affected by light intensity and photoperiod under influence of white LED light. Protected Horticulture and Plant Factory 22:228-233.
10.12791/KSBEC.2013.22.3.228
20
Saebo A., T. Krekling, and M. Appelgren. 1995. Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro. Plant Cell Tissue Organ Cult. 41:177-185.
10.1007/BF00051588
21
Samuolienė, G., R. Sirtautas, A. Brazaitytė, J. Sakalauskaitė, S. Sakalauskienė, and P. Duchovskis. 2011. The impact of red and blue light-emitting diode illumination on radish physiological indices. Central Eur. J. Biol. 6:821-828.
10.2478/s11535-011-0059-z
22
Savvides A., D. Fanourakis, and W. van Leperen. 2012. Coordination of hydraulic and stomatal conductances across light qualities in cucumber leaves. J. Exp. Bot. 63:1135-1143.
10.1093/jxb/err34822121201PMC3276089
23
Son K.H., J.H. Park, D. Kim, and M.M. Oh. 2012. Leaf shape, growth, and phytochemicals in two leaf lettuce cultivars grown under monochromatic light-emitting diodes. Korean J. Hortic. Sci. Technol. 30:664-672.
10.7235/hort.2012.12063
24
Son K.H., and M.M. Oh. 2015. Growth, photosynthetic and antioxidant parameters of two lettuce cultivars as affected by red, green, and blue light-emitting diodes. Hortic. Environ. Biotechnol. 56:639-653.
10.1007/s13580-015-1064-3
25
Son, K.H., Y.M. Jeon, and M.M. Oh. 2016. Application of Supplementary White and Pulsed Light-emitting Diodes to Lettuce Grown in a Plant Factory with Artificial Lighting. Hortic. Environ. Biotechnol. 57:560-572.
10.1007/s13580-016-0068-y
26
Taiz L., and E. Zeiger. 1991. Plant physiology. 1st ed., 179-264. Benjamin Cummings Publishing Co. New York.
27
Tamulaitis G, P. Duchovskis, Z. Bliznikas, K. Breive, R. Ulinskaite, A. Brazaityte, A. Novickovas, and A. Zukauskas. 2005. High-power light-emitting diode based facility for plant cultivation. J. Phys. D Appl Phys 38:293-3187.
10.1088/0022-3727/38/17/S20
28
US Department of Energy 2011. Solid-state lighting research and development: multi year program plan (Fig. 3.4), p. 130.
29
XiaoYing, L., G. ShiRong, X. ZhiGang, J. XueLei, and T. Tezuka. 2011. Regulation of chloroplast ultrastructure, cross-section anatomy of leaves, and morphology of stomata of cherry tomato by different light irradiations of lightemitting diodes. HortScience 46:217-221.
10.21273/HORTSCI.46.2.217
30
Yoneda K, and Y. Mori. 2004 Method of cultivating plant and illuminator for cultivating plant. European Patent Ofce. EP1374665A1.
Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Protected Horticulture and Plant Factory
  • Journal Title(Ko) :시설원예ㆍ식물공장
  • Volume : 26
  • No :2
  • Pages :123-132
  • Received Date : 2017-03-28
  • Revised Date : 2017-04-17
  • Accepted Date : 2017-04-25
  • DOI :https://doi.org/10.12791/KSBEC.2017.26.2.123
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