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Original Articles

Changes in Growth and Bioactive Compounds of Lettuce According to CO2 Tablet Treatment in the Nutrient Solution of Hydroponic System

수경재배 양액 내 탄산정 처리에 의한 상추의 생육 및 생리활성물질 함량 변화

Gwonjeong Bok1
,
Seungwon Noh1
,
Youngkuk Kim2
,
Changsu Nam3
,
Chaelin Jin3
,
Jongseok Park4†
복 권정1
,
노 승원1
,
김 영국2
,
남 창수3
,
진 채린3
,
박 종석4†
1Graduate Student, Department of Horticulture Science, College of Agriculture & Life Sciences, Chungnam National University, Deajeon 34134, Korea
2Professor, Department of Computer Science and Engineering, College of Engineering, Chungnam National University, Deajeon 34134, Korea
3Under Graduate Student, Department of Horticulture Science, College of Agriculture & Life Sciences, Chungnam National University, Deajeon 34134, Korea
4Professor, Department of Horticulture Science, College of Agriculture & Life Sciences, Chungnam National University, Deajeon 34134, Korea
1충남대학교 농업생명과학대학 원예학과 대학원생
2충남대학교 공과대학 컴퓨터융합학부 교수
3충남대학교 농업생명과학대학 원예학과 학부생
4충남대학교 농업생명과학대학 원예학과 교수
*jongseok@cnu.ac.kr
31 January 2021. pp. 85-93
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Abstract

In hydroponic cultivation, in order to investigate the change of lettuce growth and physiologically active substances through CO2 tablet treatment in nutrient solution, we used a solid carbonated tablets commercially available in the Netherlands. The experiment consisted of 0.5-fold, 1-fold, and 2-fold treatment groups with no treatment as a control. As a result, the atmospheric CO2 concentration in the chamber after CO2 tablet treatment showed the highest value at 472.2 µL·L-1 in the 2-fold treatment zone immediately after treatment, and the pH in the nutrient solution decreased the most to pH 6.03 in the 2-fold treatment zone. After that, over time, the CO2 concentration and pH recovered to the level before treatment. Leaf width and leaf area of lettuce showed the highest values of 17.1cm and 1067.14 cm2 when treated 2-fold with CO2 tablet, while fresh weight and dry weight of the above-ground part were highest at 63.87 g and 3.08 g in 0.5-fold treatment. The root length of lettuce was the longest (28.4 cm) in the control, but there was no significant difference in the fresh weight and the dry weight among the treatments. Apparently, it was observed that the root length of the lettuce was shortened by CO2 tablet treatment and a lot of side roots occurred. In addition, there was a growth disorder in which the roots turned black, but it was found that there was no negative effect on the growth of the above-ground part. As a result of analyzing the bioactive compounds of lettuce by CO2 tablet treatment, chlorogenic acid and quercetin were detected. As a result of quantitative analysis, chlorogenic acid increased by 249% compared to the control in 1-fold treatment, but quercetin decreased by 37%. As a result of comparing the DPPH radical scavenging ability showing antioxidant activity, the control and 0.5-fold treatment showed significantly higher values than the 1-fold and 2-fold treatments. This suggests that carbonated water treatment is effective in increasing the growth and bioactive compounds of hydroponic lettuce.

Keywords
antioxidant activity
chlorogenic acid
DFT
DPPH
HPLC
quercetin

수경재배시 양액 내 탄산정 처리를 통한 상추의 생육 및 생리활성물질 변화를 조사하기 위해 네덜란드에서 시판되는 고형 탄산정을 사용하였다. 실험은 무처리를 대조구로 하여 0.5배, 1배, 2배 처리구로 구성하였다. 실험결과, 탄산정 처리 후 챔버내 대기 CO2 농도는 처리 직후 2배 처리구에서 472.2µL·L-1로 가장 높은 수치를 보였으며, 양액내 pH는 2배 처리구는 pH 6.03로 가장 많이 감소하였다. 이후 시간이 경과함에 따라 CO2 농도와 pH는 처리 전 수준으로 회복하는 모습을 나타냈다. 상추의 엽폭과 엽면적은 탄산정 2배 처리시 17.1cm, 1067cm2로 가장 큰 값을 나타내었으며 지상부 생체중, 건물중은 0.5배 처리구에서 63.87g, 3.08g으로 가장 높게 나타났다. 상추의 근장은 대조구에서 28.4cm로 가장 길었으나 처리구들간에 지하부의 생체중, 건물중은 유의적인 차이를 나타내지 않았다. 외관상 탄산정 처리에 의해 상추의 근장이 짧아지고 곁뿌리가 많이 발생한 것이 관찰되었다. 또한 뿌리가 갈색으로 약간 변하는 결과가 있었지만, 지상부 생육에는 부정적인 영향을 미치지 않은 것으로 나타났다. 탄산정 처리에 의한 상추의 생리활성물질을 분석한 결과 chlorogenic acid와 quercetin 두가지 물질이 검출되었으며 이를 정량분석한 결과 1배 처리구에서 chlrogenic acid는 대조구보다 249% 증가하였지만 quercetin은 37% 감소한 결과를 나타냈다. 항산화 활성을 나타내는 DPPH 라디컬 소거능을 비교한 결과 대조구와 0.5배 처리가 1배, 2배 처리보다 유의적으로 높은 값을 나타냈다. 이를 통해 탄산정 처리가 수경재배 상추의 생육과 생리활성물질을 증대에 효과가 있음을 제시한다.

키워드
chlorogenic acid
DPPH
quercetin
담액수경
액체크로마토그래피
항산화 활성
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Information
  • Publisher :The Korean Society for Bio-Environment Control
  • Publisher(Ko) :(사)한국생물환경조절학회
  • Journal Title :Journal of Bio-Environment Control
  • Journal Title(Ko) :생물환경조절학회지
  • Volume : 30
  • No :1
  • Pages :85-93
  • Received Date : 2021-01-15
  • Revised Date : 2021-01-25
  • Accepted Date : 2021-01-25
  • DOI :https://doi.org/10.12791/KSBEC.2021.30.1.085
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